PMID- 10720473
OWN - NLM
STAT- MEDLINE
DCOM- 20000419
LR  - 20221207
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 269
IP  - 3
DP  - 2000 Mar 24
TI  - Expression of monocyte chemoattractant protein-1 by nonenzymatically glycated 
      albumin (Amadori adducts) in vascular smooth muscle cells.
PG  - 666-70
AB  - The biological effects of Amadori adducts that are early nonenzymatically 
      glycated protein on vascular cells were poorly defined. We examined the effect of 
      glycated serum albumin (GA) on the expression of monocyte chemoattractant 
      protein-1(MCP-1) that is an important chemokine recruiting monocyte to blood 
      vessel. GA increased MCP-1 mRNA expression with a peak after 3 h of stimulation. 
      The induction of MCP-1 by GA was dose-dependent. The MCP-1 mRNA expression by GA 
      was completely inhibited by PD98059 and genistein that inhibit mitogen activated 
      protein (MAP) kinase kinase and tyrosine kinase, respectively. N-Acetylcysteine, 
      a potent antioxidant, also suppressed the GA-induced MCP-1 expression. These 
      results suggest that GA induces production of reactive oxygen species and 
      activates tyrosine kinase and MAP kinase in VSMC. Activation of these signals 
      results in MCP-1 expression. GA-induced MCP-1 expression may be one of the 
      mechanisms by which the diabetic patients suffer from accelerated 
      atherosclerosis.
CI  - Copyright 2000 Academic Press.
FAU - Ichiki, T
AU  - Ichiki T
AD  - Department of Cardiovascular Medicine, Kyushu University Graduate School of 
      Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. 
      ichiki@cardiol.med.kyushu-u.ac.jp
FAU - Funakoshi, Y
AU  - Funakoshi Y
FAU - Ito, K
AU  - Ito K
FAU - Takeshita, A
AU  - Takeshita A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (RNA, Messenger)
RN  - 0 (Serum Albumin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
RN  - 0 (Glycated Serum Albumin)
SB  - IM
MH  - Animals
MH  - Aorta, Thoracic/metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics
MH  - Enzyme Inhibitors/pharmacology
MH  - Flavonoids/pharmacology
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Glycation End Products, Advanced
MH  - Kinetics
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Muscle, Smooth, Vascular/*metabolism
MH  - Protein-Tyrosine Kinases/metabolism
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serum Albumin/*pharmacology
MH  - Serum Albumin, Bovine/pharmacology
MH  - Transcription, Genetic
MH  - Glycated Serum Albumin
EDAT- 2000/03/18 09:00
MHDA- 2000/04/25 09:00
CRDT- 2000/03/18 09:00
PHST- 2000/03/18 09:00 [pubmed]
PHST- 2000/04/25 09:00 [medline]
PHST- 2000/03/18 09:00 [entrez]
AID - S0006-291X(00)92350-7 [pii]
AID - 10.1006/bbrc.2000.2350 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Mar 24;269(3):666-70. doi: 
      10.1006/bbrc.2000.2350.