PMID- 10720866
OWN - NLM
STAT- MEDLINE
DCOM- 20000501
LR  - 20180213
IS  - 0302-2838 (Print)
IS  - 0302-2838 (Linking)
VI  - 37
IP  - 3
DP  - 2000 Mar
TI  - Chromosomal numerical aberrations detected by fluorescence in situ hybridization 
      on bladder washings from patients with bladder cancer.
PG  - 358-65
AB  - OBJECTIVE: Previous studies on touch biopsy specimens have determined numerical 
      or structural changes involving many different chromosomes in bladder cancer. The 
      aim of this study was to evaluate the use of fluorescence in situ hybridization 
      (FISH) assay in bladder washings as an objective technique to detect chromosomal 
      numerical aberrations in bladder cancer. The main advantages of bladder washings 
      are that they can be easily collected during the clinical follow-up of patients 
      with superficial bladder cancer and they do not contain so many degenerate cells 
      as urine samples. METHODS: We collected specimens from 25 patients who underwent 
      transurethral resection of bladder tumors. Double target FISH assays with 
      centromeric labeled probes for chromosomes 7, 8, 9 and 11 were used on the 
      bladder washings and on the touch biopsy slides. The results were compared to 
      flow cytometry and tumor grade and stage. RESULTS: We found monosomy 9 and 
      trisomy 7, 8, 9 and 11 in 28, 32, 36, 28 and 25% respectively of the patients. 
      FISH analysis of bladder washing versus touch biopsy specimens were concordant in 
      approximately 90% of the slides. Total DNA aneuploidy correlated well with 
      numerical aberrations of chromosomes 7, 8 and 11, but not with chromosome 9. 
      CONCLUSION: Although better hybridization efficiency was obtained on touch biopsy 
      slides, the results in bladder washings were in high concordance. FISH analysis 
      on bladder washing samples may become a simple tool to improve the accuracy of 
      cytology.
FAU - Marano, A
AU  - Marano A
AD  - Department of Urology, Karolinska Hospital, Stockholm, Sweden. 
      alex_marano@hotmail.com
FAU - Pan, Y
AU  - Pan Y
FAU - Li, C
AU  - Li C
FAU - Pagliarulo, A
AU  - Pagliarulo A
FAU - Elmberger, G
AU  - Elmberger G
FAU - Tribukait, B
AU  - Tribukait B
FAU - Ekman, P
AU  - Ekman P
FAU - Bergerheim, U
AU  - Bergerheim U
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Switzerland
TA  - Eur Urol
JT  - European urology
JID - 7512719
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aneuploidy
MH  - Biopsy
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 7
MH  - Chromosomes, Human, Pair 8
MH  - Chromosomes, Human, Pair 9
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Specimen Handling
MH  - Therapeutic Irrigation
MH  - Urinary Bladder/*pathology
MH  - Urinary Bladder Neoplasms/*genetics/pathology
EDAT- 2000/03/18 09:00
MHDA- 2000/05/08 09:00
CRDT- 2000/03/18 09:00
PHST- 2000/03/18 09:00 [pubmed]
PHST- 2000/05/08 09:00 [medline]
PHST- 2000/03/18 09:00 [entrez]
AID - 52370 [pii]
AID - 10.1159/000052370 [doi]
PST - ppublish
SO  - Eur Urol. 2000 Mar;37(3):358-65. doi: 10.1159/000052370.