PMID- 10720946
OWN - NLM
STAT- MEDLINE
DCOM- 20000518
LR  - 20061115
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 57
IP  - 3
DP  - 2000 Mar
TI  - Inhibition of monocyte chemoattractant protein-1 expression in tubular epithelium 
      attenuates tubulointerstitial alteration in rat Goodpasture syndrome.
PG  - 927-36
AB  - BACKGROUND: To examine the role of monocyte chemoattractant protein-1 (MCP-1) 
      expressed by tubular epithelium in tubulointerstitial alterations in situ, the 
      level of MCP-1 mRNA in tubular epithelium was lowered selectively in the rat 
      model of Goodpasture syndrome (GPS). METHODS: Intravenously administered 
      antisense oligodeoxynucleotide (ODN) is taken up by renal tubular epithelium and 
      has been found to block expression of target genes in rats. MCP-1 antisense ODN 
      was injected into GPS rats every second day from days 27 to 35 after immunization 
      (this represents the time when renal MCP-1 mRNA level was increased and 
      interstitial mononuclear cell infiltration was aggravated). RESULTS: In addition 
      to a reduction in the level of tubular MCP-1 mRNA, antisense ODN treatment 
      attenuated monocyte infiltration significantly and preserved renal function in 
      GPS rats. However, ODN injection did not affect glomerular MCP-1 expression and 
      glomerular histopathology, and there were no significant changes in the urinary 
      protein excretion rate. CONCLUSION: Our findings provide direct evidence that 
      MCP-1, expressed by tubular epithelium, plays a pivotal role in mediating 
      secondary tubulointerstitial alterations in the GPS model.
FAU - Okada, H
AU  - Okada H
AD  - Department of Nephrology, Saitama Medical College, Japan.
FAU - Moriwaki, K
AU  - Moriwaki K
FAU - Kalluri, R
AU  - Kalluri R
FAU - Imai, H
AU  - Imai H
FAU - Ban, S
AU  - Ban S
FAU - Takahama, M
AU  - Takahama M
FAU - Suzuki, H
AU  - Suzuki H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Chemokine CCL2)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Anti-Glomerular Basement Membrane Disease/*metabolism/pathology/physiopathology
MH  - Chemokine CCL2/*antagonists & inhibitors/genetics/metabolism
MH  - Epithelium/metabolism
MH  - Kidney/metabolism/pathology/physiopathology
MH  - Kidney Tubules/*metabolism
MH  - Male
MH  - Oligonucleotides, Antisense/pharmacology
MH  - RNA, Messenger/antagonists & inhibitors/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Distribution
EDAT- 2000/03/18 09:00
MHDA- 2000/05/20 09:00
CRDT- 2000/03/18 09:00
PHST- 2000/03/18 09:00 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 2000/03/18 09:00 [entrez]
AID - S0085-2538(15)46825-5 [pii]
AID - 10.1046/j.1523-1755.2000.00909.x [doi]
PST - ppublish
SO  - Kidney Int. 2000 Mar;57(3):927-36. doi: 10.1046/j.1523-1755.2000.00909.x.