PMID- 10722692
OWN - NLM
STAT- MEDLINE
DCOM- 20000427
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 12
DP  - 2000 Mar 24
TI  - JAB1 interacts with both the progesterone receptor and SRC-1.
PG  - 8540-8
AB  - JAB1 (Jun activation domain-binding protein-1) has previously been described as a 
      coactivator of AP1 transcription factor. We show here, by yeast and mammalian 
      two-hybrid analyses and by pull-down experiments, that JAB1 also interacts with 
      both the progesterone receptor (PR) and the steroid receptor coactivator 1 
      (SRC-1) and that it stabilizes PR-SRC-1 complexes. We also show that JAB1 
      potentiates the activity of a variety of transcription factors known to associate 
      with SRC-1 (nuclear receptors, activator protein-1, and nuclear factor kappaB). 
      This occurs without any modification of PR or SRC-1 concentration. JAB1 is a 
      subunit of a large multiprotein complex that has been called the COP9 
      signalosome. The latter is present in plant and animal cells and has been shown 
      to be involved in a variety of cellular mechanisms including transcription 
      regulation, cell cycle control, and phosphorylation cascades. We now show that it 
      is also involved in the mechanisms of action of nuclear receptors and of their 
      coactivators.
FAU - Chauchereau, A
AU  - Chauchereau A
AD  - INSERM U 135 Hormones, Genes et Reproduction, Hopital de Bicetre, 78 rue du 
      General Leclerc, 94275 Le Kremlin Bicetre, France.
FAU - Georgiakaki, M
AU  - Georgiakaki M
FAU - Perrin-Wolff, M
AU  - Perrin-Wolff M
FAU - Milgrom, E
AU  - Milgrom E
FAU - Loosfelt, H
AU  - Loosfelt H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
RN  - EC 3.4.- (Peptide Hydrolases)
SB  - IM
MH  - Binding Sites
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Histone Acetyltransferases
MH  - Nuclear Receptor Coactivator 1
MH  - Peptide Hydrolases
MH  - Protein Binding
MH  - Receptors, Cytoplasmic and Nuclear/metabolism
MH  - Receptors, Glucocorticoid/metabolism
MH  - Receptors, Progesterone/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Signal Transduction
MH  - Transcription Factor AP-1/metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Two-Hybrid System Techniques
EDAT- 2000/03/18 09:00
MHDA- 2000/04/29 09:00
CRDT- 2000/03/18 09:00
PHST- 2000/03/18 09:00 [pubmed]
PHST- 2000/04/29 09:00 [medline]
PHST- 2000/03/18 09:00 [entrez]
AID - S0021-9258(18)30158-3 [pii]
AID - 10.1074/jbc.275.12.8540 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Mar 24;275(12):8540-8. doi: 10.1074/jbc.275.12.8540.