PMID- 10725716
OWN - NLM
STAT- MEDLINE
DCOM- 20000504
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 164
IP  - 7
DP  - 2000 Apr 1
TI  - Large-scale culture and selective maturation of human Langerhans cells from 
      granulocyte colony-stimulating factor-mobilized CD34+ progenitors.
PG  - 3600-7
AB  - Dendritic cells (DCs) play a critical role as APCs in the induction of the 
      primary immune response. Their capacity for Ag processing and presentation is 
      tightly regulated, controlled by a terminal developmental sequence accompanied by 
      striking changes in morphology, organization, and function. The maturation 
      process, which converts DCs from cells adapted for Ag accumulation to cells 
      adapted for T cell stimulation, remains poorly understood due in part to 
      difficulties in the culture and manipulation of DCs of defined lineages. To 
      address these issues, we have devised conditions for the culture of a single DC 
      type, Langerhans cells (LCs), using CD34+ cells from G-CSF-mobilized patients. 
      Homogenous populations of LCs, replete with abundant immunocytochemically 
      demonstrable Birbeck granules, could be stably maintained as immature DCs for 
      long periods in culture. Unlike other human DC preparations, the LCs remained 
      fully differentiated after cytokine removal. Following exposure to TNF-alpha, 
      LPS, or CD40 ligand, the LCs could be synchronously induced to mature. Depending 
      on the agent used, distinct types of LCs emerged differing in their capacity for 
      T cell stimulation, IL-12 production, intracellular localization of MHC products, 
      and overall morphology. Most interestingly, the expression of different sets of 
      Toll family receptors is induced or down-regulated according to the maturation 
      stimulus provided. These results strongly suggest that different proinflammatory 
      stimuli might drive distinct developmental events.
FAU - Gatti, E
AU  - Gatti E
AD  - Department of Cell Biology, Ludwig Institute for Cancer Research, New Haven, CT 
      06520, USA.
FAU - Velleca, M A
AU  - Velleca MA
FAU - Biedermann, B C
AU  - Biedermann BC
FAU - Ma, W
AU  - Ma W
FAU - Unternaehrer, J
AU  - Unternaehrer J
FAU - Ebersold, M W
AU  - Ebersold MW
FAU - Medzhitov, R
AU  - Medzhitov R
FAU - Pober, J S
AU  - Pober JS
FAU - Mellman, I
AU  - Mellman I
LA  - eng
GR  - K08-AI01493/AI/NIAID NIH HHS/United States
GR  - R01-HL43364/HL/NHLBI NIH HHS/United States
GR  - R01-HL51014/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, CD1)
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CTAGE1 protein, human)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 147205-72-9 (CD40 Ligand)
SB  - IM
MH  - Adult
MH  - Antigens, CD1/biosynthesis
MH  - Antigens, CD34/*biosynthesis
MH  - Antigens, Differentiation, T-Lymphocyte
MH  - Antigens, Neoplasm
MH  - CD40 Ligand
MH  - Cell Count
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation/drug effects/immunology
MH  - Dendritic Cells/cytology/immunology/metabolism
MH  - Granulocyte Colony-Stimulating Factor/*physiology
MH  - Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Immunophenotyping
MH  - Langerhans Cells/*cytology/*immunology/metabolism
MH  - Leukapheresis
MH  - Ligands
MH  - Lipopolysaccharides/pharmacology
MH  - Membrane Glycoproteins/biosynthesis/pharmacology
MH  - Stem Cells/*cytology/*immunology/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2000/03/22 09:00
MHDA- 2000/05/08 09:00
CRDT- 2000/03/22 09:00
PHST- 2000/03/22 09:00 [pubmed]
PHST- 2000/05/08 09:00 [medline]
PHST- 2000/03/22 09:00 [entrez]
AID - ji_v164n7p3600 [pii]
AID - 10.4049/jimmunol.164.7.3600 [doi]
PST - ppublish
SO  - J Immunol. 2000 Apr 1;164(7):3600-7. doi: 10.4049/jimmunol.164.7.3600.