PMID- 10727280
OWN - NLM
STAT- MEDLINE
DCOM- 20000509
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 62
IP  - 4
DP  - 2000 Apr
TI  - Human villous macrophage-conditioned media enhance human trophoblast growth and 
      differentiation in vitro.
PG  - 1075-83
AB  - In human chorionic villi, numerous macrophages, so-called Hofbauer cells, are 
      located adjacent to trophoblasts. To determine the role of the macrophages in the 
      proliferation and differentiation of trophoblasts, cytotrophoblast cells were 
      cultured in serum-free culture-conditioned media of villous macrophages (VMCM), 
      peritoneal macrophages (PMCM), and villous fibroblasts (VFCM). In VMCM, 
      proliferation of cytotrophoblast cells was detected at 24 h by 
      immunocytochemistry with Ki-67-antibody. A large number (P < 0.001) of 
      multinucleated syncytia was formed in VMCM. In VMCM, cytotrophoblast cell fusion 
      was completed by 96 h, which coincided with the peak of hCG secretion and 
      initiation of human placental lactogen (hPL) release. Levels of hCG (P < 0.001) 
      and hPL (P < 0. 001) secretion from syncytial cells were significantly higher in 
      VMCM than in PMCM or in VFCM. Concentrations of macrophage colony-stimulating 
      factor (M-CSF) and vascular endothelial growth factor (VEGF) analyzed by ELISA 
      were greater in VMCM than in PMCM or in VFCM, whereas monocyte chemoattractant 
      protein-1 (MCP-1) concentration was high in PMCM. The expression patterns of 
      M-CSF, VEGF, and MCP-1 in villous macrophages and peritoneal macrophages by 
      reverse transcriptase-polymerase chain reaction were similar to their secretion 
      patterns. Thus, villous macrophages have a greater ability to stimulate hCG and 
      hPL secretion than do peritoneal macrophages. This study suggests that 
      macrophages within the villous stroma may stimulate the growth and 
      differentiation of trophoblasts through their secreted substances.
FAU - Khan, S
AU  - Khan S
AD  - Department of Obstetrics and Gynecology, Kumamoto University School of Medicine, 
      Kumamoto-City 860-8556, Japan.
FAU - Katabuchi, H
AU  - Katabuchi H
FAU - Araki, M
AU  - Araki M
FAU - Nishimura, R
AU  - Nishimura R
FAU - Okamura, H
AU  - Okamura H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (DNA Primers)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 9035-54-5 (Placental Lactogen)
SB  - IM
MH  - Cell Differentiation/physiology
MH  - Chemokine CCL2/metabolism
MH  - Chorion/cytology
MH  - Chorionic Gonadotropin/metabolism
MH  - Culture Media, Conditioned
MH  - DNA Primers
MH  - Endothelial Growth Factors/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fibroblasts
MH  - Humans
MH  - Immunohistochemistry
MH  - Lymphokines/metabolism
MH  - Macrophage Colony-Stimulating Factor/metabolism
MH  - Macrophages/metabolism/*physiology
MH  - Macrophages, Peritoneal/physiology
MH  - Placental Lactogen/metabolism
MH  - Pregnancy
MH  - Radioimmunoassay
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trophoblasts/metabolism/*physiology
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 2000/03/22 09:00
MHDA- 2000/05/16 09:00
CRDT- 2000/03/22 09:00
PHST- 2000/03/22 09:00 [pubmed]
PHST- 2000/05/16 09:00 [medline]
PHST- 2000/03/22 09:00 [entrez]
AID - 10.1095/biolreprod62.4.1075 [doi]
PST - ppublish
SO  - Biol Reprod. 2000 Apr;62(4):1075-83. doi: 10.1095/biolreprod62.4.1075.