PMID- 10727540
OWN - NLM
STAT- MEDLINE
DCOM- 20000517
LR  - 20221207
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 15
IP  - 4
DP  - 2000 Apr
TI  - Oxidative stress occurs in absence of hyperglycaemia and inflammation in the 
      onset of kidney lesions in normotensive obese rats.
PG  - 467-76
AB  - BACKGROUND: Several factors favour the development of kidney lesions. We examined 
      the role of oxidative stress in the onset of renal alterations that occur in 
      Zucker obese (ZO) fa/fa rats. METHODS: Kidney structure, biological data, 
      glycation parameters, advanced glycation end products (AGE), thiobarbituric 
      acid-reactive substances (TBARS), circulating antibodies anti-malondialdehyde 
      (MDA)-modified low-density lipoprotein (LDL), antioxidant defenses (Cu/Zn and Mn 
      superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) activities, 
      glutathione level), were determined in plasma and/or kidney of young and old ZO 
      rats and lean (ZL) Fa/fa littermates. RESULTS: Renal lesions and functional 
      decline appeared at 3 months in hyperlipidaemic, hyperinsulinaemic, normotensive 
      ZO rats, independently of any macrophage-ED(1)(+)-cell infiltration. At 6 months 
      and thereafter, kidney lesions and functional impairment worsened while numerous 
      ED(1)(+)-cells invaded the interstitium. At 3 and 9 months, TBARS level in the 
      LDL/very low-density lipoprotein fraction and in the kidney was higher in ZO than 
      in ZL rats. Anti-MDA-LDL antibodies were increased in ZO rats. At 3 months, renal 
      activity of Cu/Zn SOD was higher, and activities of catalase and GPx lower in ZO 
      than in ZL rats, leading to an accumulation of hydrogen peroxide (H(2)O(2)). At 9 
      months, a decrease in Cu/Zn SOD activity and an increase in glutathione level 
      were observed. Blood glucose and glycated proteins, as well as AGE in kidney, 
      remained similar in both ZL and ZO rats, whatever their age. CONCLUSION: These 
      data suggest that oxidative stress triggers, at an early age, the onset of kidney 
      lesions and functional impairment in ZO rats, in absence of hyperglycaemia, 
      hypertension and inflammation.
FAU - Poirier, B
AU  - Poirier B
AD  - INSERM U 430, Broussais Hospital, and Claude Bernard Association, Paris, France.
FAU - Lannaud-Bournoville, M
AU  - Lannaud-Bournoville M
FAU - Conti, M
AU  - Conti M
FAU - Bazin, R
AU  - Bazin R
FAU - Michel, O
AU  - Michel O
FAU - Bariety, J
AU  - Bariety J
FAU - Chevalier, J
AU  - Chevalier J
FAU - Myara, I
AU  - Myara I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Blood Glucose)
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Blood Pressure/*physiology
MH  - Cholesterol/blood
MH  - Disease Models, Animal
MH  - Fibrosis
MH  - Glomerulosclerosis, Focal Segmental/*metabolism/pathology/physiopathology
MH  - Glycated Hemoglobin/metabolism
MH  - Glycation End Products, Advanced/metabolism
MH  - Glycosylation
MH  - Hyperglycemia/*blood
MH  - Inflammation
MH  - Kidney Tubules/metabolism/pathology
MH  - Lipid Peroxidation/physiology
MH  - Male
MH  - Oxidative Stress/*physiology
MH  - Rats
MH  - Rats, Zucker
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - Triglycerides/blood
MH  - Urodynamics
EDAT- 2000/03/23 09:00
MHDA- 2000/05/20 09:00
CRDT- 2000/03/23 09:00
PHST- 2000/03/23 09:00 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 2000/03/23 09:00 [entrez]
AID - 10.1093/ndt/15.4.467 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2000 Apr;15(4):467-76. doi: 10.1093/ndt/15.4.467.