PMID- 10727739
OWN - NLM
STAT- MEDLINE
DCOM- 20000531
LR  - 20190831
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 120
IP  - 1
DP  - 2000 Mar 15
TI  - Increased expression of endogenous biotin, but not BDNF, in telencephalic song 
      regions during zebra finch vocal learning.
PG  - 113-23
AB  - Brain-derived neurotrophic factor (BDNF) is thought to regulate multiple aspects 
      of brain development and neural plasticity in vertebrates. We have examined BDNF 
      expression in two telencephalic nuclei (RA and HVC) in the zebra finch brain that 
      control song learning by juvenile males and the production of already-learned 
      song by adults. Using two different antibody-labeling techniques (avidin-biotin 
      complex and horseradish peroxidase), we were unable to detect BDNF-like 
      immunoreactivity in RA of juvenile or adult birds. BDNF-like immunoreactive 
      labeling of somata was detected in HVC, but the density of labeled cells was not 
      different between juvenile and adult birds. Immunocytochemical findings were 
      confirmed by RT-PCR for BDNF mRNA. Thus, in contrast to a previous report 
      (Akutagawa and Konishi, Proc. Natl. Acad. Sci. USA 95 (1998) 11429-11434), we did 
      not observe elevated levels of BDNF immunoreactivity in RA and HVC of juvenile 
      birds that were learning to sing. However, RA and HVC of juvenile birds were 
      found to express elevated levels of endogenous biotin (as detected by avidin 
      peroxidase), suggesting a possible role for biotin-regulated mechanisms in 
      songbird vocal learning.
FAU - Johnson, F
AU  - Johnson F
AD  - Department of Psychology and Program in Neuroscience, Florida State University, 
      Tallahassee, FL, USA. johnson@psy.fsu.edu
FAU - Norstrom, E
AU  - Norstrom E
FAU - Soderstrom, K
AU  - Soderstrom K
LA  - eng
GR  - R01 DC002035/DC/NIDCD NIH HHS/United States
GR  - DC02035-07/DC/NIDCD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 6SO6U10H04 (Biotin)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Antibodies
MH  - Auditory Perception/physiology
MH  - Biotin/*biosynthesis
MH  - Brain-Derived Neurotrophic Factor/analysis/*genetics/immunology
MH  - Discrimination Learning/*physiology
MH  - Female
MH  - Gene Expression
MH  - Horseradish Peroxidase
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Neuronal Plasticity/genetics
MH  - RNA, Messenger/analysis
MH  - Sex Factors
MH  - Songbirds/*physiology
MH  - Telencephalon/anatomy & histology/growth & development/*metabolism
MH  - Vocalization, Animal/*physiology
EDAT- 2000/03/23 09:00
MHDA- 2000/06/03 09:00
CRDT- 2000/03/23 09:00
PHST- 2000/03/23 09:00 [pubmed]
PHST- 2000/06/03 09:00 [medline]
PHST- 2000/03/23 09:00 [entrez]
AID - S016538060000002X [pii]
AID - 10.1016/s0165-3806(00)00002-x [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 2000 Mar 15;120(1):113-23. doi: 
      10.1016/s0165-3806(00)00002-x.