PMID- 10727840
OWN - NLM
STAT- MEDLINE
DCOM- 20000504
LR  - 20190831
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 72
IP  - 3-4
DP  - 2000 Mar 15
TI  - Differentiation between high and low virulence Staphylococcus aureus strains from 
      rabbits by randomly amplified polymorphic DNA (RAPD) analysis.
PG  - 311-9
AB  - Randomly Amplified Polymorphic DNA (RAPD) typing was performed on 53 rabbit 
      Staphylococcus aureus strains. Twenty-three strains isolated in 13 different 
      rabbitries with chronic problems of staphylococcosis, showed the same RAPD 
      banding pattern. Twenty of these strains belonged to the 'mixed CV-C' biotype and 
      to the phage-type 3A/3C/55/71, previously described to be highly virulent in 
      rabbits, and three strains belonged to other biotypes or phage-types. None of the 
      strains isolated from rabbitries without chronic problems of staphylococcosis 
      showed this specific RAPD pattern. RAPD analysis can be used as a rapid and 
      reliable test method to differentiate between the characteristic genotype 
      corresponding to high virulence and other S. aureus strains from rabbits. This is 
      useful for the diagnosis and prevention of the introduction of these highly 
      virulent strains in industrial rabbitries.
FAU - Hermans, K
AU  - Hermans K
AD  - Department of Pathology, Bacteriology and Poultry diseases, Faculty of Veterinary 
      Medicine, University of Ghent, Salisburylaan 133, 9820, Merelbeke, Belgium. 
      katleen.hermans@rug.ac.be
FAU - Haesebrouck, F
AU  - Haesebrouck F
FAU - Vaneechoutte, M
AU  - Vaneechoutte M
FAU - Devriese, L A
AU  - Devriese LA
FAU - Godard, C
AU  - Godard C
FAU - De Herdt, P
AU  - De Herdt P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Animals
MH  - DNA Primers/chemistry
MH  - DNA, Bacterial/chemistry/isolation & purification
MH  - Disease Outbreaks/veterinary
MH  - Electrophoresis, Agar Gel
MH  - Humans
MH  - Photography/veterinary
MH  - Polymerase Chain Reaction/veterinary
MH  - Rabbits/*microbiology
MH  - Random Amplified Polymorphic DNA Technique/*veterinary
MH  - Staphylococcal Infections/microbiology/prevention & control/*veterinary
MH  - Staphylococcus aureus/classification/genetics/*pathogenicity
MH  - Virulence
EDAT- 2000/03/23 09:00
MHDA- 2000/05/08 09:00
CRDT- 2000/03/23 09:00
PHST- 2000/03/23 09:00 [pubmed]
PHST- 2000/05/08 09:00 [medline]
PHST- 2000/03/23 09:00 [entrez]
AID - S0378-1135(99)00197-2 [pii]
AID - 10.1016/s0378-1135(99)00197-2 [doi]
PST - ppublish
SO  - Vet Microbiol. 2000 Mar 15;72(3-4):311-9. doi: 10.1016/s0378-1135(99)00197-2.