PMID- 10727905
OWN - NLM
STAT- MEDLINE
DCOM- 20000519
LR  - 20220310
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 466
IP  - 2
DP  - 2000 Mar 23
TI  - The modifying effect of beta-carotene on gamma radiation-induced elevation of 
      oxidative reactions and genotoxicity in male rats.
PG  - 179-86
AB  - The aim of the present work was to evaluate the modulatory role of beta-carotene 
      on the radiation-induced changes in certain biochemical and cytogenetic 
      parameters. beta-Carotene was given by gavage at a dose of 5 mg/kg body weight 
      for 7 consecutive days before whole body gamma irradiation with 7 Gy (single 
      dose). The levels of beta-carotene in plasma, thiobarbituric acid-reactive 
      substances (TBARS) in plasma and liver, the activities of superoxide dismutase 
      (SOD) and catalase in blood and liver were the selected parameters. Furthermore, 
      the frequency of micronuclei (MN) of polychromatic erythrocytes (PCEs), 
      normochromatic erythrocytes (NCEs), the ratio of PCEs/NCEs and the mitotic index 
      (MI) of bone marrow cells were also evaluated. The biochemical and cytogenetic 
      determinations were carried out 1, 24, and 72 h after radiation exposure. The 
      results obtained revealed that administration of beta-carotene pre-irradiation 
      significantly inhibited the decrease in plasma beta-carotene, significantly 
      reduced the levels of TBARS in plasma and liver. Significant protection of the 
      radiation-induced changes in the activities of SOD and catalase was also recorded 
      in the blood and liver of beta-carotene-treated and -irradiated rats. 
      beta-Carotene resulted in significant inhibition in the frequency of 
      radiation-induced MN, as well as in the ratio of PCEs/NCEs and the MI of bone 
      marrow cells. These results suggest that beta-carotene as a natural product with 
      its antioxidant capacity and capability of quenching singlet oxygen, could play a 
      modulatory role against the cellular damage affected by free radicals induced by 
      whole body irradiation.
FAU - El-Habit, O H
AU  - El-Habit OH
AD  - Zoology Department, Faculty of Science, Helwan University, Cairo, Egypt.
FAU - Saada, H N
AU  - Saada HN
FAU - Azab, K S
AU  - Azab KS
FAU - Abdel-Rahman, M
AU  - Abdel-Rahman M
FAU - El-Malah, D F
AU  - El-Malah DF
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Antioxidants)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 01YAE03M7J (beta Carotene)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Bone Marrow Cells/drug effects/metabolism/radiation effects
MH  - Catalase/blood/metabolism
MH  - Erythrocytes/drug effects/metabolism/radiation effects
MH  - Gamma Rays
MH  - Lipid Peroxidation/*drug effects/radiation effects
MH  - Liver/drug effects/enzymology/radiation effects
MH  - Male
MH  - Micronuclei, Chromosome-Defective/*drug effects/radiation effects
MH  - Mitotic Index
MH  - Mutagenicity Tests
MH  - Rats
MH  - Superoxide Dismutase/blood/metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - Time Factors
MH  - beta Carotene/blood/*pharmacology
EDAT- 2000/03/23 09:00
MHDA- 2000/06/08 09:00
CRDT- 2000/03/23 09:00
PHST- 2000/03/23 09:00 [pubmed]
PHST- 2000/06/08 09:00 [medline]
PHST- 2000/03/23 09:00 [entrez]
AID - S1383571800000103 [pii]
AID - 10.1016/s1383-5718(00)00010-3 [doi]
PST - ppublish
SO  - Mutat Res. 2000 Mar 23;466(2):179-86. doi: 10.1016/s1383-5718(00)00010-3.