PMID- 10730586
OWN - NLM
STAT- MEDLINE
DCOM- 20000425
LR  - 20220321
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 8
IP  - 1
DP  - 2000
TI  - Localization of repetitive DNAs to zebrafish (Danio rerio) chromosomes by 
      fluorescence in situ hybridization (FISH).
PG  - 27-35
AB  - The genome of the zebrafish, Danio rerio, contains two major classes of tandem 
      repetitive elements (AT-rich and GC-rich). The AT-rich repeats can be further 
      subdivided into two subgroups which differ by about 10% of 185 bp in the 
      repeating unit. The chromosomal location of these sequences and the moderately 
      repetitive 5S rDNA sequences was determined in two diploid zebrafish cell lines 
      using in-situ hybridization with fluorochrome-labeled probes. The AT-rich 
      sequences were found at the centromeres of all chromosome pairs and the GC-rich 
      sequences were found in paracentromeric location on over half of the chromosomal 
      pairs. Different patterns of hybridization were found for the two subgroups of 
      the AT-rich family. One type hybridized primarily to centromeres of one half to 
      two thirds of the chromosomal pairs and the other type to centromeres of about 
      three fourths of the chromosomal pairs. The pattern of hybridization with the 
      GC-rich sequences varied somewhat between the cell lines consistent with 
      interindividual variation in the location of paracentromeric heterochromatin. The 
      5S rRNA genes are found on the long arm of chromosome 3. Most of this chromosome 
      arm is late replicating, but apparently does not contain either the AT-rich or 
      GC-rich repetitive sequences.
FAU - Phillips, R B
AU  - Phillips RB
AD  - Department of Biological Sciences, University of Wisconsin-Milwaukee, 53201, USA. 
      rp@csd.uwm.edu
FAU - Reed, K M
AU  - Reed KM
LA  - eng
GR  - GM/OD54317/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - AT Rich Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA Probes
MH  - DNA, Ribosomal
MH  - GC Rich Sequence
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - RNA, Ribosomal, 5S/genetics
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Zebrafish/*genetics
EDAT- 2000/03/24 09:00
MHDA- 2000/04/29 09:00
CRDT- 2000/03/24 09:00
PHST- 2000/03/24 09:00 [pubmed]
PHST- 2000/04/29 09:00 [medline]
PHST- 2000/03/24 09:00 [entrez]
AID - 10.1023/a:1009271017998 [doi]
PST - ppublish
SO  - Chromosome Res. 2000;8(1):27-35. doi: 10.1023/a:1009271017998.