PMID- 10732025
OWN - NLM
STAT- MEDLINE
DCOM- 20000405
LR  - 20190822
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 49
IP  - 2
DP  - 1998 Jan 15
TI  - Activation of bovine and baboon primordial follicles in vitro.
PG  - 441-9
AB  - Mammalian ovaries contain a large pool of non-growing, primordial follicles. The 
      ability to initiate growth of this pool of resting follicles in vitro and to 
      maintain follicular growth to a stage when the oocyte could be matured and 
      fertilized would increase the reproductive potential of valuable domestic 
      animals, endangered species and infertile women. This paper summarizes our 
      progress to date in activating primordial follicles of cattle and baboons. Pieces 
      of ovarian cortex, rich in primordial follicles, were obtained from fetal bovine 
      and baboon ovaries during late gestation. Pieces were maintained in organ culture 
      in serum-free medium containing ITS+ (insulin-transferrin-selenium-linoleic 
      acid-BSA) for up to 20 days and at various times during culture some pieces were 
      fixed for histological morphometry. As early as 2 days of culture, the number of 
      primordial follicles had decreased by 88% or 55%, whereas the number of primary 
      follicles had increased 2.5- or 5-fold, compared to tissue freshly isolated from 
      bovine or baboon ovaries, respectively (P < 0.01). In baboon cortical pieces a 
      small number of secondary follicles developed during a 20-day culture period. The 
      development of primary and secondary follicles was accompanied by an increase in 
      diameter of both the granulosa cell layer and the oocyte. The addition of FSH (1, 
      10, or 100 ng/ml) had no effect on the development of follicles in bovine 
      cortical pieces after 7 or 14 days of culture, relative to control cultures 
      without FSH. These results show that a high percentage of primordial follicles 
      from cattle and baboons can be activated to grow in serum-free medium in the 
      absence of gonadotropins. Conditions that will support further growth in vitro of 
      follicles from these species remain to be elucidated. The culture system we have 
      developed could be used to develop such conditions and to explore factors that 
      regulate the movement of primordial follicles into the pool of growing follicles.
FAU - Fortune, J E
AU  - Fortune JE
AD  - Department of Physiology, College of Veterinary Medicine, Cornell University, 
      Ithaca, NY 14853, USA.
FAU - Kito, S
AU  - Kito S
FAU - Wandji, S A
AU  - Wandji SA
FAU - Srsen, V
AU  - Srsen V
LA  - eng
GR  - HD21939/HD/NICHD NIH HHS/United States
GR  - HD35168/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Size
MH  - Female
MH  - Fetus
MH  - Gestational Age
MH  - Oocytes/*cytology
MH  - Organ Culture Techniques
MH  - Ovarian Follicle/*cytology/embryology/*physiology
MH  - Ovary/cytology/*embryology
MH  - Papio
EDAT- 2000/03/25 00:00
MHDA- 2000/03/25 00:01
CRDT- 2000/03/25 00:00
PHST- 2000/03/25 00:00 [pubmed]
PHST- 2000/03/25 00:01 [medline]
PHST- 2000/03/25 00:00 [entrez]
AID - S0093691X97004160 [pii]
AID - 10.1016/s0093-691x(97)00416-0 [doi]
PST - ppublish
SO  - Theriogenology. 1998 Jan 15;49(2):441-9. doi: 10.1016/s0093-691x(97)00416-0.