PMID- 10732294
OWN - NLM
STAT- MEDLINE
DCOM- 20000411
LR  - 20181113
IS  - 0169-4197 (Print)
IS  - 0169-4197 (Linking)
VI  - 26
IP  - 3
DP  - 1999 Dec
TI  - Reduced basal and stimulated leukocyte adherence in tumor endothelium of 
      experimental pancreatic cancer.
PG  - 173-9
AB  - BACKGROUND: The interaction between immunocompetent cells and tumor endothelium 
      is essential for an effective immunological response. In the present study, we 
      evaluated resting and CD11b/CD18-mediated leukocyte adhesion in tumor vessels of 
      experimental pancreatic cancer and in healthy pancreatic venules in the rat. 
      METHODS: Solid tumor fragments (1 mm3) were interposed intrapancreatically 
      between inert transparent polymethylmetacrylate plates for intravital microscopy 
      (n = 12) by which tumor microcirculation, leukocyte-tumor-endothelium 
      interaction, and the effect of the chemoattractants 
      N-formyl-methioninleucylphenylalanine (fMLP) and platelet-activating factor (PAF) 
      on leukocyte adherence was investigated. RESULTS: Leukocyte adhesion in 
      pancreatic tumor vessels was significantly reduced compared to healthy pancreatic 
      venules. Both fMLP and PAF dramatically increased leukocyte adherence in normal 
      pancreatic venules. No change in leukocyte adhesion was present in tumor vessels 
      after exposure to these chemotactic substances. CONCLUSION: Resting and 
      stimulated integrin-dependent leukocyte adhesion is strongly reduced in malignant 
      vessels of experimental pancreatic cancer, which may be an important mechanism to 
      escape immune control.
FAU - Schmidt, J
AU  - Schmidt J
AD  - Department of Surgery, University of Heidelberg, Germany.
FAU - Ryschich, E
AU  - Ryschich E
FAU - Maksan, S M
AU  - Maksan SM
FAU - Werner, J
AU  - Werner J
FAU - Gebhard, M M
AU  - Gebhard MM
FAU - Herfarth, C
AU  - Herfarth C
FAU - Klar, E
AU  - Klar E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Pancreatol
JT  - International journal of pancreatology : official journal of the International 
      Association of Pancreatology
JID - 8703511
RN  - 0 (Chemotactic Factors)
RN  - 0 (Platelet Activating Factor)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Animals
MH  - Blood Flow Velocity
MH  - Blood Vessels/metabolism
MH  - Carcinoma/blood supply/*pathology/*physiopathology
MH  - Cell Adhesion/drug effects/physiology
MH  - Chemotactic Factors/pharmacology
MH  - Endothelium/*physiopathology
MH  - Endothelium, Vascular/physiopathology
MH  - Erythrocytes/physiology
MH  - Leukocytes/*physiology
MH  - Lymphocyte Culture Test, Mixed
MH  - N-Formylmethionine Leucyl-Phenylalanine/metabolism
MH  - Pancreatic Neoplasms/blood supply/*pathology/*physiopathology
MH  - Platelet Activating Factor/metabolism
MH  - Rats
MH  - Venules
EDAT- 2000/03/25 09:00
MHDA- 2000/04/15 09:00
CRDT- 2000/03/25 09:00
PHST- 2000/03/25 09:00 [pubmed]
PHST- 2000/04/15 09:00 [medline]
PHST- 2000/03/25 09:00 [entrez]
AID - 10.1385/IJGC:26:3:173 [doi]
PST - ppublish
SO  - Int J Pancreatol. 1999 Dec;26(3):173-9. doi: 10.1385/IJGC:26:3:173.