PMID- 10733340
OWN - NLM
STAT- MEDLINE
DCOM- 20000317
LR  - 20131121
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 73
IP  - 4
DP  - 1999 Jun 15
TI  - Effects of serum on calcium mobilization in the submandibular cell line A253.
PG  - 458-68
AB  - The effects of serum on inositol 1,4,5-trisphosphate (IP3) formation and Ca2+ 
      mobilization in the human submandibular cell line A253 were studied. Exposure of 
      A253 cells to fetal bovine serum (FBS) elicited a 3.3-fold increase in IP3 
      formation and a concentration-dependent transient increase in cytosolic free Ca2+ 
      concentration ([Ca2+]i), which was similar in Ca2+-containing and Ca2+-free 
      media. Newborn bovine serum (NBS), but not bovine serum albumin (BSA), induced a 
      similar response. The Ca2+ release triggered by FBS was significantly (88%) 
      reduced by the phospholipase C inhibitor U73122, indicating that Ca2+ release 
      induced by FBS is through the PLC pathway. Pretreatment with the tyrosine kinase 
      inhibitor genistein abolished the FBS- and NBS-induced Ca2+ release, suggesting 
      that tyrosine kinase plays an important role in mediating the Ca2+ release. 
      Pre-exposure to ATP or thapsigargin (TG) significantly reduced the FBS-induced 
      [Ca2+]i increase, indicating that Ca2+ release caused by FBS is from the TG- or 
      ATP-sensitive Ca2+ store. While FBS exposure elicited a large Ca2+ release, it 
      reduced Ca2+ influx. Furthermore, FBS significantly inhibited the Ca2+ influx 
      activated by the depletion of intracellular stores by ATP or TG. These results 
      suggest that (1) serum elicits Ca2+ release from ATP- and TG-sensitive stores, 
      which is mediated by IP3; (2) the serum-induced Ca2+ release may be modulated by 
      a tyrosine kinase-associated process; and (3) serum strongly inhibits Ca2+ 
      influxes including the store depletion-activated Ca2+ influx.
FAU - Sun, X
AU  - Sun X
AD  - Department of Pediatrics, University of Texas Health Science Center at San 
      Antonio, 78284, USA.
FAU - Mork, A C
AU  - Mork AC
FAU - Helmke, R J
AU  - Helmke RJ
FAU - Martinez, J R
AU  - Martinez JR
FAU - Zhang, G H
AU  - Zhang GH
LA  - eng
GR  - DE09270/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium Signaling/physiology
MH  - Cattle
MH  - Fetal Blood/*physiology
MH  - Humans
MH  - Intracellular Fluid/metabolism
MH  - Submandibular Gland Neoplasms/*metabolism
MH  - Tumor Cells, Cultured
MH  - Type C Phospholipases/metabolism
EDAT- 2000/03/25 09:00
MHDA- 2000/06/20 09:00
CRDT- 2000/03/25 09:00
PHST- 2000/03/25 09:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 2000/03/25 09:00 [entrez]
AID - 10.1002/(SICI)1097-4644(19990615)73:4<458::AID-JCB4>3.0.CO;2-0 [pii]
PST - ppublish
SO  - J Cell Biochem. 1999 Jun 15;73(4):458-68.