PMID- 10733505
OWN - NLM
STAT- MEDLINE
DCOM- 20000427
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 95
IP  - 7
DP  - 2000 Apr 1
TI  - In vitro growth inhibition of a broad spectrum of tumor cell lines by activated 
      human dendritic cells.
PG  - 2346-51
AB  - Dendritic cells (DCs) are critical subsets of leukocytes providing antigen 
      presentation for initiation of humoral and cellular immune responses. Their role 
      as effector cells in tumor resistance, however, is less known. We report here 
      that human DCs generated by culturing plastic-adherent peripheral blood monocytes 
      in the presence of granulocyte-monocyte colony-stimulating factor (GM-CSF) and 
      interleukin-4 have potent growth-inhibition activity in vitro on a wide spectrum 
      of human tumor lines of different tissue origin. Proinflammatory stimuli 
      lipopolysaccharide (LPS) and interferon-gamma, but not tumor necrosis 
      factor-alpha and CD40 signaling, can further enhance DC-mediated inhibition of 
      tumor growth. The growth inhibition requires contact between DCs and tumor cells 
      while LPS treatment enhances the antitumor activity in DC culture supernatants. 
      Our results suggest that in addition to their predominant role as regulatory 
      cells, activated DCs are also potential effector cells in tumor immunity.
FAU - Chapoval, A I
AU  - Chapoval AI
AD  - Department of Immunology, Mayo Graduate and Medical Schools, Mayo Clinic, 
      Rochester, MN, USA.
FAU - Tamada, K
AU  - Tamada K
FAU - Chen, L
AU  - Chen L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plastics)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Communication
MH  - *Cell Division
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Immunity, Innate
MH  - Interleukin-4/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/cytology/immunology
MH  - Neoplasms/*immunology/*pathology
MH  - Plastics
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/physiology
EDAT- 2000/03/25 09:00
MHDA- 2000/04/29 09:00
CRDT- 2000/03/25 09:00
PHST- 2000/03/25 09:00 [pubmed]
PHST- 2000/04/29 09:00 [medline]
PHST- 2000/03/25 09:00 [entrez]
AID - S0006-4971(20)64196-9 [pii]
PST - ppublish
SO  - Blood. 2000 Apr 1;95(7):2346-51.