PMID- 10733578
OWN - NLM
STAT- MEDLINE
DCOM- 20000425
LR  - 20240314
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 20
IP  - 8
DP  - 2000 Apr
TI  - Translation of NRF mRNA is mediated by highly efficient internal ribosome entry.
PG  - 2755-9
AB  - The ubiquitous transcription factor NRF (NF-kappaB repressing factor) is a 
      constitutive transcriptional silencer of the multifunctional cytokine 
      interferon-beta. NRF mRNA contains a long 5' untranslated region (5'UTR) 
      predicted to fold into a strong secondary structure. The presence of stable 
      hairpins is known to be incompatible with efficient translation by ribosomal 
      scanning. Using dicistronic reporter gene constructs, we show that the NRF 5'UTR 
      acts as an internal ribosome entry site (IRES) which directs ribosomes to the 
      downstream start codon by a cap-independent mechanism. The relative activity of 
      this IRES in various cell lines is at least 30-fold higher than that of 
      picornaviral IRESs. The NRF 5'UTR also functions as a translational enhancer in 
      the context of monocistronic mRNAs. Our results indicate that the NRF 5'UTR 
      contains a highly potent IRES, which may allow for an alternate mode of 
      translation under physiological conditions in which cap-dependent translation is 
      inhibited.
FAU - Oumard, A
AU  - Oumard A
AD  - Department of Gene Regulation and Differentiation, National Research Institute 
      for Biotechnology, D-38124 Braunschweig, Germany.
FAU - Hennecke, M
AU  - Hennecke M
FAU - Hauser, H
AU  - Hauser H
FAU - Nourbakhsh, M
AU  - Nourbakhsh M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NKRF protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - 3T3 Cells
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Cricetinae
MH  - *DNA-Binding Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Plasmids
MH  - *Protein Biosynthesis
MH  - RNA, Messenger/*genetics
MH  - Repressor Proteins/*genetics
MH  - Ribosomes/*genetics
MH  - Transcription Factors/*genetics
MH  - Transfection
PMC - PMC85491
EDAT- 2000/03/25 09:00
MHDA- 2000/04/29 09:00
PMCR- 2000/04/01
CRDT- 2000/03/25 09:00
PHST- 2000/03/25 09:00 [pubmed]
PHST- 2000/04/29 09:00 [medline]
PHST- 2000/03/25 09:00 [entrez]
PHST- 2000/04/01 00:00 [pmc-release]
AID - 1754 [pii]
AID - 10.1128/MCB.20.8.2755-2759.2000 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2000 Apr;20(8):2755-9. doi: 10.1128/MCB.20.8.2755-2759.2000.