PMID- 10734153
OWN - NLM
STAT- MEDLINE
DCOM- 20000421
LR  - 20190617
IS  - 0022-3565 (Print)
IS  - 0022-3565 (Linking)
VI  - 293
IP  - 1
DP  - 2000 Apr
TI  - Neuromuscular dysfunction in the jejunum and colon of human leukocyte antigen B27 
      transgenic rats.
PG  - 60-6
AB  - HLA-B27 transgenic rats are a model of spontaneous gastrointestinal inflammation 
      associated with expression of human leukocyte antigen (HLA) B27 and 
      beta(2)-microglobulin. Our goal was to investigate in vitro enteric nerve 
      regulation and contractile activity in isolated longitudinal muscles from the 
      jejunum and colon of HLA-B27 rats. Nontransgenic age-matched Fisher 344 rats were 
      used as controls. Intestinal inflammation and tissue injury, quantified 
      histologically and through tissue myeloperoxidase activity, were evident in both 
      the jejunum and colon of HLA-B27 rats. Although resting tension and spontaneous 
      activity of the jejunal and colonic muscles from HLA-B27 rats did not differ 
      significantly from controls, responses to both enteric nerve stimulation or 
      direct muscle activation were significantly inhibited. In muscles from HLA-B27 
      rats, electrical field stimulation (0.5 ms, 0.5-20 Hz) induced low-amplitude 
      contractions (maximal reduction 60-65%) compared with respective controls. In the 
      presence of atropine and guanethidine, nonadrenergic and noncholinergic 
      contractile responses to higher frequencies of stimulation (8-20 Hz) were also of 
      lower amplitude. These changes were accompanied by a shift in neurally mediated 
      contractions from predominantly cholinergic in the jejunum and colon of Fisher 
      344 rats to predominantly nonadrenergic and noncholinergic in HLA-B27 rats. 
      Furthermore, maximal contractions to carbachol or KCl depolarization were reduced 
      (up to 2.7-fold) compared with respective controls. In the jejunum of HLA-B27 
      rats the EC(50) level for carbachol was decreased. The data indicate that 
      gastrointestinal inflammation induced by expression of HLA-B27 is associated with 
      hypocontractility and inhibition of enteric cholinergic control of the 
      longitudinal muscle in both the small and large intestine.
FAU - Venkova, K
AU  - Venkova K
AD  - Oklahoma Foundation for Digestive Research Basic Science Laboratories, Veterans 
      Affairs Medical Center, Oklahoma City, Oklahoma, USA.
FAU - Dunn, S T
AU  - Dunn ST
FAU - Adesina, A M
AU  - Adesina AM
FAU - Greenwood-Van Meerveld, B
AU  - Greenwood-Van Meerveld B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pharmacol Exp Ther
JT  - The Journal of pharmacology and experimental therapeutics
JID - 0376362
RN  - 0 (Adrenergic Agents)
RN  - 0 (HLA-B27 Antigen)
RN  - 0 (Muscarinic Agonists)
RN  - 0 (Muscarinic Antagonists)
RN  - 660YQ98I10 (Potassium Chloride)
RN  - 7C0697DR9I (Atropine)
RN  - 8Y164V895Y (Carbachol)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - ZTI6C33Q2Q (Guanethidine)
SB  - IM
MH  - Adrenergic Agents/pharmacology
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Atropine/pharmacology
MH  - Carbachol/pharmacology
MH  - Colitis/*genetics/*physiopathology
MH  - Electric Stimulation
MH  - Enteritis/*genetics/*physiopathology
MH  - Guanethidine/pharmacology
MH  - HLA-B27 Antigen/*genetics
MH  - In Vitro Techniques
MH  - Jejunal Diseases/*genetics/*physiopathology
MH  - Male
MH  - Muscarinic Agonists/pharmacology
MH  - Muscarinic Antagonists/pharmacology
MH  - Muscle, Smooth/pathology
MH  - Neuromuscular Diseases/*genetics
MH  - Parasympathetic Nervous System/physiopathology
MH  - Peroxidase/metabolism
MH  - Potassium Chloride/pharmacology
MH  - Rats
MH  - Rats, Inbred F344
EDAT- 2000/03/29 09:00
MHDA- 2000/04/29 09:00
CRDT- 2000/03/29 09:00
PHST- 2000/03/29 09:00 [pubmed]
PHST- 2000/04/29 09:00 [medline]
PHST- 2000/03/29 09:00 [entrez]
PST - ppublish
SO  - J Pharmacol Exp Ther. 2000 Apr;293(1):60-6.