PMID- 10735087 OWN - NLM STAT- MEDLINE DCOM- 20000426 LR - 20190822 IS - 0093-691X (Print) IS - 0093-691X (Linking) VI - 52 IP - 8 DP - 1999 Dec TI - Clinical experience with flow cytometric separation of human X- and Y-chromosome bearing sperm. PG - 1435-40 AB - Numerous methods to separate human X- and Y-bearing sperm have been reported with unconfirmed separation after DNA analysis and inconsistent birth results. Successful flow cytometric separation of sperm resulting in alteration of the sex ratio of young born has been demonstrated in several animal species. Flow cytometric separation of human X- and Y-bearing sperm (MicroSort) has been confirmed after DNA analysis by fluorescence in situ hybridization (FISH). Pregnancies and births have resulted from the use of MicroSort after intrauterine insemination (IUI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). FAU - Fugger, E F AU - Fugger EF AD - Genetics & IVF Institute, Fairfax, VA, USA. LA - eng PT - Journal Article PL - United States TA - Theriogenology JT - Theriogenology JID - 0421510 RN - 9007-49-2 (DNA) SB - IM MH - Animals MH - DNA/analysis MH - Female MH - Humans MH - In Situ Hybridization, Fluorescence MH - Male MH - Reproductive Techniques MH - Sex Determination Analysis/*methods MH - Sex Preselection/*methods MH - Sex Ratio MH - Spermatozoa/*cytology MH - X Chromosome MH - Y Chromosome EDAT- 2000/03/29 09:00 MHDA- 2000/04/29 09:00 CRDT- 2000/03/29 09:00 PHST- 2000/03/29 09:00 [pubmed] PHST- 2000/04/29 09:00 [medline] PHST- 2000/03/29 09:00 [entrez] AID - S0093691X99002289 [pii] AID - 10.1016/s0093-691x(99)00228-9 [doi] PST - ppublish SO - Theriogenology. 1999 Dec;52(8):1435-40. doi: 10.1016/s0093-691x(99)00228-9.