PMID- 10735593
OWN - NLM
STAT- MEDLINE
DCOM- 20000616
LR  - 20190921
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 43
IP  - 3
DP  - 2000 Mar
TI  - Establishment and epitope analysis of allo-specific cytotoxic T lymphocytes at a 
      tumor site recognizing a spouse's HLA-A0206 molecule.
PG  - 167-73
AB  - PROBLEM: The molecular basis of allo-reactivity in reproductive immunity has not 
      been fully clarified. METHOD OF STUDY: Cytotoxic T lymphocytes (CTLs) were 
      established from tumor-infiltrating lymphocytes (TILs). The allo-reactivity of 
      the CTLs against various tumor cell lines or human leukocyte antigen (HLA)-A 
      allele-transfected COS-7 cells was measured by 51Cr-release or interferon-gamma 
      production assay. RESULTS AND CONCLUSIONS: We have established CTLs reacting to 
      an HLA-A0206 molecule that matched a spouse's HLA-A allele from the TILs of a 
      68-year-old multiparous patient with gastric cancer. The amino acids at positions 
      66 and 88 in the alpha1 domain of HLA-A0206, both of which were common in the 
      other HLA-A2 subtypes, were involved in the recognition by the CTLs. Endogenous 
      peptides in the groove were not involved in the recognition. These results 
      suggest the presence of long-lasting memory CTLs raised by the reproduction 
      process, and may facilitate a better understanding of the molecular basis of 
      allo-recognition during reproduction.
FAU - Seki, N
AU  - Seki N
AD  - Department of Immunology, Kurume University School of Medicine, Japan.
FAU - Yamada, A
AU  - Yamada A
FAU - Suefuji, Y
AU  - Suefuji Y
FAU - Mine, T
AU  - Mine T
FAU - Tanaka, S
AU  - Tanaka S
FAU - Gomi, S Y
AU  - Gomi SY
FAU - Kawagoe, N
AU  - Kawagoe N
FAU - Koufuji, K
AU  - Koufuji K
FAU - Itoh, K
AU  - Itoh K
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A2 Antigen)
SB  - IM
MH  - Aged
MH  - Alleles
MH  - Animals
MH  - COS Cells
MH  - Epitopes, T-Lymphocyte/*analysis
MH  - Female
MH  - HLA-A2 Antigen/genetics/*immunology/metabolism
MH  - Humans
MH  - Immunologic Memory
MH  - Lymphocytes, Tumor-Infiltrating/immunology
MH  - Male
MH  - *Spouses
MH  - Stomach Neoplasms/*immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology/metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 2000/03/29 09:00
MHDA- 2000/06/24 11:00
CRDT- 2000/03/29 09:00
PHST- 2000/03/29 09:00 [pubmed]
PHST- 2000/06/24 11:00 [medline]
PHST- 2000/03/29 09:00 [entrez]
AID - 10.1111/j.8755-8920.2000.430306.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2000 Mar;43(3):167-73. doi: 
      10.1111/j.8755-8920.2000.430306.x.