PMID- 10735862
OWN - NLM
STAT- MEDLINE
DCOM- 20000418
LR  - 20210526
IS  - 0021-9193 (Print)
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Linking)
VI  - 182
IP  - 8
DP  - 2000 Apr
TI  - Roles of horizontal gene transfer and gene integration in evolution of 
      1,3-dichloropropene- and 1,2-dibromoethane-degradative pathways.
PG  - 2191-9
AB  - The haloalkane-degrading bacteria Rhodococcus rhodochrous NCIMB13064, Pseudomonas 
      pavonaceae 170, and Mycobacterium sp. strain GP1 share a highly conserved 
      haloalkane dehalogenase gene (dhaA). Here, we describe the extent of the 
      conserved dhaA segments in these three phylogenetically distinct bacteria and an 
      analysis of their flanking sequences. The dhaA gene of the 
      1-chlorobutane-degrading strain NCIMB13064 was found to reside within a 
      1-chlorobutane catabolic gene cluster, which also encodes a putative invertase 
      (invA), a regulatory protein (dhaR), an alcohol dehydrogenase (adhA), and an 
      aldehyde dehydrogenase (aldA). The latter two enzymes may catalyze the oxidative 
      conversion of n-butanol, the hydrolytic product of 1-chlorobutane, to n-butyric 
      acid, a growth substrate for many bacteria. The activity of the dhaR gene product 
      was analyzed in Pseudomonas sp. strain GJ1, in which it appeared to function as a 
      repressor of dhaA expression. The 1,2-dibromoethane-degrading strain GP1 
      contained a conserved DNA segment of 2.7 kb, which included dhaR, dhaA, and part 
      of invA. A 12-nucleotide deletion in dhaR led to constitutive expression of dhaA 
      in strain GP1, in contrast to the inducible expression of dhaA in strain 
      NCIMB13064. The 1, 3-dichloropropene-degrading strain 170 possessed a conserved 
      DNA segment of 1.3 kb harboring little more than the coding region of the dhaA 
      gene. In strains 170 and GP1, a putative integrase gene was found next to the 
      conserved dhaA segment, which suggests that integration events were responsible 
      for the acquisition of these DNA segments. The data indicate that horizontal gene 
      transfer and integrase-dependent gene acquisition were the key mechanisms for the 
      evolution of catabolic pathways for the man-made chemicals 1, 3-dichloropropene 
      and 1,2-dibromoethane.
FAU - Poelarends, G J
AU  - Poelarends GJ
AD  - Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology 
      Institute, University of Groningen, 9747 AG Groningen, The Netherlands.
FAU - Kulakov, L A
AU  - Kulakov LA
FAU - Larkin, M J
AU  - Larkin MJ
FAU - van Hylckama Vlieg, J E
AU  - van Hylckama Vlieg JE
FAU - Janssen, D B
AU  - Janssen DB
LA  - eng
SI  - GENBANK/AJ150371
SI  - GENBANK/AJ250372
SI  - GENBANK/L49435
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Allyl Compounds)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (DhaR protein, E coli)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Hydrocarbons, Halogenated)
RN  - 0 (Trans-Activators)
RN  - 1N41638RNO (Ethylene Dibromide)
RN  - 9H780918D0 (1,3-dichloro-1-propene)
RN  - EC 2.7.7.- (Integrases)
RN  - EC 3.- (Hydrolases)
RN  - EC 3.8.1.5 (haloalkane dehalogenase)
SB  - IM
MH  - Allyl Compounds/metabolism
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Biodegradation, Environmental
MH  - Conserved Sequence
MH  - DNA Transposable Elements
MH  - DNA-Binding Proteins/metabolism
MH  - Environmental Pollutants/metabolism
MH  - *Escherichia coli Proteins
MH  - Ethylene Dibromide/metabolism
MH  - *Evolution, Molecular
MH  - Gene Expression Regulation, Bacterial
MH  - *Gene Transfer, Horizontal
MH  - *Genes, Bacterial
MH  - Hydrocarbons, Brominated
MH  - Hydrocarbons, Chlorinated
MH  - Hydrocarbons, Halogenated/*metabolism
MH  - Hydrolases/*genetics
MH  - Integrases/genetics
MH  - Molecular Sequence Data
MH  - Mycobacterium/enzymology/genetics
MH  - Pseudomonas/enzymology/genetics
MH  - *Recombination, Genetic
MH  - Rhodococcus/enzymology/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Trans-Activators/metabolism
PMC - PMC111268
EDAT- 2000/03/29 09:00
MHDA- 2000/04/25 09:00
PMCR- 2000/04/01
CRDT- 2000/03/29 09:00
PHST- 2000/03/29 09:00 [pubmed]
PHST- 2000/04/25 09:00 [medline]
PHST- 2000/03/29 09:00 [entrez]
PHST- 2000/04/01 00:00 [pmc-release]
AID - 1538 [pii]
AID - 10.1128/JB.182.8.2191-2199.2000 [doi]
PST - ppublish
SO  - J Bacteriol. 2000 Apr;182(8):2191-9. doi: 10.1128/JB.182.8.2191-2199.2000.