PMID- 10735902
OWN - NLM
STAT- MEDLINE
DCOM- 20000421
LR  - 20170210
IS  - 0732-183X (Print)
IS  - 0732-183X (Linking)
VI  - 18
IP  - 7
DP  - 2000 Apr
TI  - Fluorescence in situ hybridization on peripheral-blood specimens is a reliable 
      method to evaluate cytogenetic response in chronic myeloid leukemia.
PG  - 1533-8
AB  - PURPOSE: To evaluate the usefulness of fluorescence in situ hybridization (FISH) 
      on peripheral-blood specimens to evaluate the cytogenetic response to treatment 
      in patients with chronic myeloid leukemia (CML). PATIENTS AND METHODS: In a first 
      attempt, we analyzed 62 bone marrow specimens using interphase FISH and compared 
      the results with those of conventional cytogenetics. In a second step, we 
      analyzed 60 paired sets of bone marrow and peripheral-blood specimens with 
      interphase FISH. RESULTS: The results of interphase FISH agreed with conventional 
      cytogenetics on bone marrow for most patients, and only minor differences were 
      found (r =.98). The comparison of interphase FISH on bone marrow versus 
      peripheral-blood specimens showed a strong correlation between these two specimen 
      sources (r =.97). CONCLUSION: Our results confirmed that FISH is a sensitive 
      technique for the evaluation of response to treatment in patients with CML. 
      Moreover, our study suggests that follow-up of cytogenetic response to therapy 
      can be evaluated on peripheral-blood specimens, thus enabling an easier and more 
      frequent evaluation of patients. The next step will be to evaluate this technique 
      in a large prospective trial to define the prognostic value of complete 
      remissions evaluated by FISH.
FAU - Le Gouill, S
AU  - Le Gouill S
AD  - Laboratory of Hematology and Clinical Hematology Department, University Hospital, 
      Nantes, France.
FAU - Talmant, P
AU  - Talmant P
FAU - Milpied, N
AU  - Milpied N
FAU - Daviet, A
AU  - Daviet A
FAU - Ancelot, M
AU  - Ancelot M
FAU - Moreau, P
AU  - Moreau P
FAU - Harousseau, J L
AU  - Harousseau JL
FAU - Bataille, R
AU  - Bataille R
FAU - Avet-Loiseau, H
AU  - Avet-Loiseau H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Oncol
JT  - Journal of clinical oncology : official journal of the American Society of 
      Clinical Oncology
JID - 8309333
SB  - IM
MH  - Blood Cells/cytology
MH  - Bone Marrow Cells/cytology
MH  - Cytogenetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy/*genetics
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Sensitivity and Specificity
MH  - *Translocation, Genetic
MH  - Treatment Outcome
EDAT- 2000/03/29 09:00
MHDA- 2000/04/29 09:00
CRDT- 2000/03/29 09:00
PHST- 2000/03/29 09:00 [pubmed]
PHST- 2000/04/29 09:00 [medline]
PHST- 2000/03/29 09:00 [entrez]
AID - 10.1200/JCO.2000.18.7.1533 [doi]
PST - ppublish
SO  - J Clin Oncol. 2000 Apr;18(7):1533-8. doi: 10.1200/JCO.2000.18.7.1533.