PMID- 10736092
OWN - NLM
STAT- MEDLINE
DCOM- 20000404
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 51
IP  - 3
DP  - 2000 Mar
TI  - Schistosoma mansoni in mice: the pattern of primary cercarial exposure determines 
      whether a secondary infection post-chemotherapy elicits a T helper 1- or a T 
      helper 2-associated immune response.
PG  - 237-43
AB  - Reinfection with Schistosoma mansoni following chemotherapy often results in an 
      ameliorated granulomatous reaction and hence a mild disease. This study examined 
      some of the immunological mechanisms that could be associated with this residual 
      protection. BALB/c mice were infected with either a single dose (group A) of 100 
      S. mansoni cercariae or with 10 doses of 10 cercariae each (group B) given at 
      3-day intervals. The mice were treated with praziquantel 8 weeks postinfection 
      and, 2 weeks later, together with another group of naive mice (group C), they 
      were infected with a single dose of 100 cercariae each. All the animals were 
      killed 8 weeks later and schistosome egg antigen (SEA)- and soluble adult worm 
      antigen preparation (SWAP)-induced cytokine recall responses in splenocytes, as 
      well as serum immunoglobulin levels, were quantified and hepatic granuloma sizes 
      measured. Group A animals had higher levels of SEA-induced interferon-gamma 
      (IFN-gamma) but lower levels of interleukin (IL)-5 than groups B and C (P < 
      0.01). Group B animals had low SEA-induced IFN-gamma levels and elevated IL-5 
      levels, although these were lower than group C. SEA-induced IL-10 was low in both 
      groups A and B as compared to group C (P < 0.01). SWAP was less effective as an 
      inducer of splenocyte cytokine production than SEA but both SWAP-induced 
      IFN-gamma and IL-5 were detected in groups A and C. SEA- and SWAP-specific 
      immunoglobulin E (IgE) and immunoglobulin G (IgG) titres were not significantly 
      different between the three groups. Granuloma diameters were larger in group C 
      (mean 297 +/- 51.3 microm) as compared to groups A (174 +/- 49 microm, P < 0.01) 
      and B (247.5 +/- 44 microm, P < 0.05). Taken together, these results demonstrate 
      that granuloma size is reduced during a reinfection exposure compared with a 
      primary infection. This reduction is associated with a T helper 1 response in 
      mice exposed to a single large dose of cercariae in the primary infection and 
      with a predominantly T helper 2 response in those infected with multiple small 
      doses.
FAU - Farah, I O
AU  - Farah IO
AD  - Division of Comparative Medicine, Department of Physiology, Biomedical Centre, 
      Uppsala University, Sweden.
FAU - Johansson, M
AU  - Johansson M
FAU - Lovgren-Bengtson, K
AU  - Lovgren-Bengtson K
FAU - Hau, J
AU  - Hau J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Culture Media)
RN  - 0 (Cytokines)
RN  - 11028-71-0 (Concanavalin A)
RN  - 6490C9U457 (Praziquantel)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/biosynthesis/blood
MH  - Antigens, Helminth/immunology/pharmacology
MH  - Cells, Cultured
MH  - Concanavalin A/pharmacology
MH  - Culture Media/pharmacology
MH  - Cytokines/biosynthesis
MH  - Female
MH  - Granuloma/immunology/parasitology/pathology
MH  - Liver Diseases, Parasitic/immunology/parasitology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Praziquantel/therapeutic use
MH  - Recurrence
MH  - Schistosoma mansoni/*growth & development/*immunology
MH  - Schistosomiasis mansoni/drug therapy/*immunology/*parasitology/pathology
MH  - Spleen/cytology
MH  - Th1 Cells/*immunology/metabolism
MH  - Th2 Cells/*immunology/metabolism
EDAT- 2000/03/29 00:00
MHDA- 2000/03/29 00:01
CRDT- 2000/03/29 00:00
PHST- 2000/03/29 00:00 [pubmed]
PHST- 2000/03/29 00:01 [medline]
PHST- 2000/03/29 00:00 [entrez]
AID - sji667 [pii]
AID - 10.1046/j.1365-3083.2000.00667.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2000 Mar;51(3):237-43. doi: 10.1046/j.1365-3083.2000.00667.x.