PMID- 10736524
OWN - NLM
STAT- MEDLINE
DCOM- 20000504
LR  - 20190915
IS  - 0145-305X (Print)
IS  - 0145-305X (Linking)
VI  - 24
IP  - 4
DP  - 2000 Jun
TI  - Cloning and analysis of expression of Mx cDNA in Japanese flounder, Paralichthys 
      olivaceus.
PG  - 407-15
AB  - Myxovirus resistance (Mx) is an interferon (IFN)-induced protein that 
      specifically prevents growth, in vivo and in vitro, of certain classes of virus. 
      We isolated the Mx cDNA from a leukocyte cDNA library obtained from a Japanese 
      flounder infected with Hirame rhabdovirus (HRV). The Mx cDNA was found to contain 
      2385 bp coding for 620 amino acids. The amino acid sequence had approximately 51 
      and 78% identities to the sequences of human and rainbow trout Mx cDNAs, 
      respectively. We propose that the amino acid sequence encoded by this cDNA clone 
      contains a tripartite guanosine-5'-triphosphate (GTP)-binding motif, GXXXSGKS/T, 
      DXXG and T/NKXD, and a signature of the dynamin family, LPRG(S/K)GIVTR, which are 
      conserved in all IFN-induced Mx proteins of vertebrates. In addition, the 
      C-terminal region of the Japanese flounder Mx protein, like those of other Mx 
      proteins, contains a highly conserved leucine zipper motif. Japanese flounder 
      infected with the HRV showed increased expression of Mx mRNA in the leukocytes 
      starting at 48 h and peaking at 72 h. An RT-PCR (reverse transcription-polymerase 
      chain-reaction) analysis of Mx mRNA from a healthy fish showed that the Mx mRNA 
      was predominantly expressed in the kidney, spleen, intestine, brain, peritoneal 
      cavity fluid and gill, and only minimally expressed in leukocytes, liver, muscle 
      and mucus.
FAU - Lee, J Y
AU  - Lee JY
AD  - Laboratory of Genetics and Biochemistry, Department of Aquatic Biosciences, Tokyo 
      University of Fisheries, Konan, Minato-ku, Tokyo, Japan.
FAU - Hirono, I
AU  - Hirono I
FAU - Aoki, T
AU  - Aoki T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Antiviral Agents)
RN  - 0 (DNA, Complementary)
RN  - 0 (Myxovirus Resistance Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antiviral Agents/*genetics
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/isolation & purification
MH  - Flounder/*metabolism
MH  - *GTP-Binding Proteins
MH  - Molecular Sequence Data
MH  - Myxovirus Resistance Proteins
MH  - Proteins/*genetics
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2000/03/29 09:00
MHDA- 2000/05/08 09:00
CRDT- 2000/03/29 09:00
PHST- 2000/03/29 09:00 [pubmed]
PHST- 2000/05/08 09:00 [medline]
PHST- 2000/03/29 09:00 [entrez]
AID - S0145-305X(99)00060-9 [pii]
AID - 10.1016/s0145-305x(99)00060-9 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2000 Jun;24(4):407-15. doi: 10.1016/s0145-305x(99)00060-9.