PMID- 10737888
OWN - NLM
STAT- MEDLINE
DCOM- 20000523
LR  - 20190910
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 50
IP  - 3
DP  - 2000 Jun 5
TI  - Effects of transforming growth factor beta1 released from biodegradable polymer 
      microparticles on marrow stromal osteoblasts cultured on poly(propylene fumarate) 
      substrates.
PG  - 452-62
AB  - Recombinant human transforming growth factor beta1 (TGF-beta1) was incorporated 
      into microparticles of blends of poly(DL-lactic-co-glycolic acid) (PLGA) and 
      poly(ethylene glycol) (PEG) to create a delivery vehicle for the growth factor. 
      The entrapment efficiency of TGF-beta1 in the microparticles containing 5% PEG 
      was 40.3 +/- 1.2% for a TGF-beta1 loading density of 6.0 ng/1 mg of 
      microparticles. For the same loading, 17.9 +/- 0.6 and 32.1 +/- 2.5% of the 
      loaded TGF-beta1 was released after 1 and 8 days, respectively, followed by a 
      plateau for the remaining 3 weeks. Rat marrow stromal cells showed a dose 
      response to TGF-beta1 released from the microparticles similar to that of added 
      TGF-beta1, indicating the activity of TGF-beta1 was retained during microparticle 
      fabrication and after TGF-beta1 release. An optimal TGF-beta1 dosage of 1.0 ng/mL 
      was determined through a 3-day dose response study for maximal alkaline 
      phosphatase (ALP) activity. The TGF-beta1 released from the microparticles loaded 
      with 6.0 ng TGF-beta1/1 mg of microparticles for the optimal dosage of TGF-beta1 
      enhanced the proliferation and osteoblastic differentiation of marrow stromal 
      cells cultured on poly(propylene fumarate) substrates. The cells showed 
      significantly increased total cell number, ALP activity, and osteocalcin 
      production with values reaching 138,700 +/- 3300 cells/cm(2), 22.8 +/- 1.5 x 
      10(-7) micromol/min/cell, and 15.9 +/- 1.5 x 10(-6) ng/cell, respectively, after 
      21 days as compared to cells cultured under control conditions without TGF-beta1. 
      These results suggest that controlled release of TGF-beta1 from the PLGA/PEG 
      blend microparticles may find applications in modulating cellular response during 
      bone healing at a skeletal defect site.
CI  - Copyright 2000 John Wiley & Sons, Inc.
FAU - Peter, S J
AU  - Peter SJ
AD  - Departments of Bioengineering and Chemical Engineering, Rice University, Houston, 
      Texas 77005-1892, USA.
FAU - Lu, L
AU  - Lu L
FAU - Kim, D J
AU  - Kim DJ
FAU - Stamatas, G N
AU  - Stamatas GN
FAU - Miller, M J
AU  - Miller MJ
FAU - Yaszemski, M J
AU  - Yaszemski MJ
FAU - Mikos, A G
AU  - Mikos AG
LA  - eng
GR  - 5T32GM08362/GM/NIGMS NIH HHS/United States
GR  - R01-AR44381/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
RN  - 0 (Biocompatible Materials)
RN  - 0 (Capsules)
RN  - 0 (Drug Implants)
RN  - 0 (Fumarates)
RN  - 0 (Polymers)
RN  - 0 (Polypropylenes)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (poly(propylene fumarate))
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials
MH  - *Capsules
MH  - Cells, Cultured
MH  - Drug Delivery Systems
MH  - Drug Implants
MH  - Fumarates
MH  - Humans
MH  - *Lactic Acid
MH  - Osteoblasts/*drug effects
MH  - *Polyethylene Glycols
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Polymers
MH  - Polypropylenes
MH  - Rats
MH  - Transforming Growth Factor beta/*pharmacology
OTO - NASA
OT  - Non-programmatic
EDAT- 2000/03/29 09:00
MHDA- 2000/06/08 09:00
CRDT- 2000/03/29 09:00
PHST- 2000/03/29 09:00 [pubmed]
PHST- 2000/06/08 09:00 [medline]
PHST- 2000/03/29 09:00 [entrez]
AID - 10.1002/(SICI)1097-4636(20000605)50:3<452::AID-JBM20>3.0.CO;2-0 [pii]
AID - 10.1002/(sici)1097-4636(20000605)50:3<452::aid-jbm20>3.0.co;2-0 [doi]
PST - ppublish
SO  - J Biomed Mater Res. 2000 Jun 5;50(3):452-62. doi: 
      10.1002/(sici)1097-4636(20000605)50:3<452::aid-jbm20>3.0.co;2-0.