PMID- 10738307
OWN - NLM
STAT- MEDLINE
DCOM- 20000516
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 28
IP  - 1
DP  - 2000 May
TI  - Simultaneous molecular karyotyping and mapping of viral DNA integration sites by 
      25-color COBRA-FISH.
PG  - 92-7
AB  - Combined binary ratio labeling (COBRA) fluorescence in situ hybridization (FISH) 
      allows 24-color FISH karyotyping of human metaphase chromosomes utilizing only 
      four fluorochromes, instead of the five required for combinatorial labeling 
      procedures. Here we show that by introduction of a fifth fluorochrome, COBRA-FISH 
      permits molecular cytogenetic mapping of viral integration sites in complex 
      karyotypes in the context of a 24-color hybridization. We were able to detect a 
      single copy of the human papillomavirus 16 in the SiHa cell line and to confirm 
      the site of integration at 13q21-31. We also demonstrate the gene mapping 
      possibility of 25-color hybridization by detecting a MYC cosmid on normal 
      metaphase chromosomes. The possibility of mapping single-copy probes in the 
      background of 24-color hybridization expands the tools for cytogenetic mapping of 
      DNA sequences and will contribute to the understanding of the role of viral 
      integration and chromosome rearrangement in virus-mediated carcinogenesis.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Szuhai, K
AU  - Szuhai K
AD  - Laboratory for Cytochemistry, Department of Molecular Cell Biology, Leiden 
      University Medical Center, Leiden, The Netherlands.
FAU - Bezrookove, V
AU  - Bezrookove V
FAU - Wiegant, J
AU  - Wiegant J
FAU - Vrolijk, J
AU  - Vrolijk J
FAU - Dirks, R W
AU  - Dirks RW
FAU - Rosenberg, C
AU  - Rosenberg C
FAU - Raap, A K
AU  - Raap AK
FAU - Tanke, H J
AU  - Tanke HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Chromosomes, Human/genetics
MH  - DNA, Viral/*genetics
MH  - Female
MH  - Humans
MH  - Image Enhancement/methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/methods
MH  - Microscopy, Fluorescence
MH  - Papillomaviridae/genetics
MH  - Papillomavirus Infections
MH  - Tumor Cells, Cultured
MH  - Tumor Virus Infections
MH  - Uterine Cervical Neoplasms
MH  - Virus Integration/*genetics
EDAT- 2000/03/30 09:00
MHDA- 2000/05/20 09:00
CRDT- 2000/03/30 09:00
PHST- 2000/03/30 09:00 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 2000/03/30 09:00 [entrez]
AID - 10.1002/(SICI)1098-2264(200005)28:1<92::AID-GCC11>3.0.CO;2-2 [pii]
AID - 10.1002/(sici)1098-2264(200005)28:1<92::aid-gcc11>3.0.co;2-2 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 May;28(1):92-7. doi: 
      10.1002/(sici)1098-2264(200005)28:1<92::aid-gcc11>3.0.co;2-2.