PMID- 10739636
OWN - NLM
STAT- MEDLINE
DCOM- 20000523
LR  - 20131121
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 162
IP  - 2
DP  - 2000 Apr
TI  - BDNF is needed for postnatal maturation of basal forebrain and neostriatum 
      cholinergic neurons in vivo.
PG  - 297-310
AB  - Neurotrophins regulate survival, neurite outgrowth, and phenotypic maturation of 
      developing neurons. Brain-derived neurotrophic factor (BDNF) can promote the 
      survival of developing cholinergic forebrain neurons in vitro and reduce their 
      degeneration following injury in adult rats. We investigated the role of 
      endogenous BDNF during postnatal development of these cholinergic neurons by 
      analyzing homozygous BDNF-deficient (-/-) mice and their littermates (+/+, +/-). 
      At P6, the number of choline acetyltransferase- (ChAT) positive neurons in the 
      medial septum was approximately 23% lower in BDNF-/- mice, although their brain 
      and body weight was normal. At P15, control (+/+) littermates had approximately 
      45% more and approximately 45% larger ChAT-positive neurons and a much denser 
      cholinergic hippocampal innervation than at P6, indicative of maturation of the 
      septohippocampal system. In BDNF-/- mice, the number, size, and 
      ChAT-immunostaining intensity of the cholinergic neurons remained the same 
      between P6 and P15 (few mice survive longer). BDNF-/- mice had about three times 
      more TUNEL-labeled (a marker of apoptosis) cells in the medial septum at P6, 
      consistent with (but not proof of) the possibility that the cholinergic neurons 
      were dying. The cholinergic hippocampal innervation in BDNF-/- mice expanded to a 
      lesser extent than in controls and had reduced levels of acetylcholinesterase 
      staining at P15. The developmental deficits were largely similar in the 
      neostriatum of BDNF-/- mice. These findings suggest that BDNF is critical for 
      postnatal development and maturation of cholinergic forebrain neurons.
CI  - Copyright 2000 Academic Press.
FAU - Ward, N L
AU  - Ward NL
AD  - Department of Anatomy and Neurobiology, Dalhousie University, Halifax, Nova 
      Scotia, B3H 4H7, Canada.
FAU - Hagg, T
AU  - Hagg T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/metabolism
MH  - Animals
MH  - Apoptosis
MH  - Body Weight
MH  - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology
MH  - Cell Count/drug effects
MH  - Choline O-Acetyltransferase/metabolism
MH  - Heterozygote
MH  - Hippocampus/cytology/drug effects/enzymology/growth & development
MH  - Homozygote
MH  - In Situ Nick-End Labeling
MH  - Mice
MH  - Mice, Knockout
MH  - Neostriatum/cytology/drug effects/enzymology/*growth & development
MH  - Nerve Fibers/drug effects/enzymology
MH  - Neurons/*cytology/drug effects/*enzymology
MH  - Septum of Brain/cytology/drug effects/enzymology/*growth & development
EDAT- 2000/03/31 09:00
MHDA- 2000/06/08 09:00
CRDT- 2000/03/31 09:00
PHST- 2000/03/31 09:00 [pubmed]
PHST- 2000/06/08 09:00 [medline]
PHST- 2000/03/31 09:00 [entrez]
AID - S0014-4886(99)97346-6 [pii]
AID - 10.1006/exnr.1999.7346 [doi]
PST - ppublish
SO  - Exp Neurol. 2000 Apr;162(2):297-310. doi: 10.1006/exnr.1999.7346.