PMID- 10740199
OWN - NLM
STAT- MEDLINE
DCOM- 20000519
LR  - 20190905
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 20
IP  - 4
DP  - 2000 Apr
TI  - Comparison of fetal cell recovery from maternal blood using a high density 
      gradient for the initial separation step: 1.090 versus 1.119 g/ml.
PG  - 281-6
AB  - The purpose of this study was to improve recovery of fetal nucleated erythrocytes 
      (NRBCs) from maternal blood for non-invasive prenatal diagnosis. Peripheral blood 
      samples were obtained from 27 women who had just undergone pregnancy termination 
      at 6 to 23 weeks. Samples were split and mononuclear cells were isolated using 
      Histopaque gradient at densities of 1.090 g/ml and 1.119 g/ml. CD45 depletion 
      using magnetic activated cell-sorting, followed by flow-sorting with antibody to 
      gamma-globin and fluorescence in situ hybridization (FISH) analysis, were used to 
      evaluate the number of fetal NRBCs recovered. In samples separated with the 1.119 
      g/ml density gradient, the yield of true anti-gamma haemoglobin positive cells 
      (median, 14. 9; range, 0-717.5) was significantly higher than that with the 1.090 
      g/ml density gradient (median, 4.9; range, 0-532.5). After FISH analysis, in the 
      14 samples in which the fetal karyotype differed from the mother, the median 
      number of fetal NRBCs separated by the 1. 119 g/ml density gradient was 22.9 
      (2-717.5), which was significantly higher than that by the 1.090 g/ml gradient 
      (median, 11.5; range, 0-532.5, p=0.022). Increased density of the gradient used 
      for the initial enrichment of fetal cells results in improved fetal cell recovery 
      in fresh post-termination blood samples, which may permit better non-invasive 
      detection of fetal cells in maternal blood.
CI  - Copyright 2000 John Wiley & Sons, Ltd.
FAU - Samura, O
AU  - Samura O
AD  - Division of Genetics, Department of Pediatrics, New England Medical Center, Tufts 
      University School of Medicine, Boston, MA 02111, USA.
FAU - Sekizawa, A
AU  - Sekizawa A
FAU - Zhen, D K
AU  - Zhen DK
FAU - Falco, V M
AU  - Falco VM
FAU - Bianchi, D W
AU  - Bianchi DW
LA  - eng
GR  - HD-4-3204/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Blood Cells/*cytology
MH  - Cell Separation/*methods
MH  - Centrifugation, Density Gradient
MH  - Erythrocytes/*cytology
MH  - Female
MH  - Flow Cytometry
MH  - Gestational Age
MH  - Humans
MH  - Immunomagnetic Separation
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Pregnancy
EDAT- 2000/03/31 09:00
MHDA- 2000/06/08 09:00
CRDT- 2000/03/31 09:00
PHST- 2000/03/31 09:00 [pubmed]
PHST- 2000/06/08 09:00 [medline]
PHST- 2000/03/31 09:00 [entrez]
AID - 10.1002/(SICI)1097-0223(200004)20:4<281::AID-PD812>3.0.CO;2-P [pii]
AID - 10.1002/(sici)1097-0223(200004)20:4<281::aid-pd812>3.0.co;2-p [doi]
PST - ppublish
SO  - Prenat Diagn. 2000 Apr;20(4):281-6. doi: 
      10.1002/(sici)1097-0223(200004)20:4<281::aid-pd812>3.0.co;2-p.