PMID- 10741094
OWN - NLM
STAT- MEDLINE
DCOM- 20000413
LR  - 20161020
IS  - 1122-0643 (Print)
IS  - 1122-0643 (Linking)
VI  - 54
IP  - 5
DP  - 1999 Oct
TI  - Lymphocyte expression of human leukocyte antigen class II molecules in patients 
      with chronic obstructive pulmonary disease.
PG  - 384-9
AB  - Chronic obstructive pulmonary disease (COPD) is a multifactorial disorder, 
      deriving from a combination of environmental and genetic factors. Polymorphisms 
      of genes of the human major histocompatibility complex in COPD have been poorly 
      studied in the past. In a preliminary approach, it was difficult to type human 
      leukocyte antigen (HLA) at the protein level and it was hypothesized that there 
      was a reduced surface density of HLA class II molecules. The aims of this study 
      were to analyse, by cytofluorimetry, HLA class I and II molecule densities on 
      peripheral mononuclear cells of COPD patients and to investigate whether there 
      was a correlation with the polymorphisms of DQA and DQB promoter regions which 
      are supposed to be important factors involved in surface expression of HLA-DQ 
      molecules. The study investigated 27 male COPD patients admitted because of 
      disease exacerbation and 49 healthy male controls. Quantitative analysis of 
      fluorescence intensity of HLA class I (A, B, C) and class II (DR, DP, DQ) 
      molecules was performed on blood mononuclear cells by cytoron cytofluorimetry. 
      Polymorphisms of DQA and DQB promoters (QAP and QBP) were determined from the DNA 
      (PCR-SSO). The surface densities of HLA class I and HLA-DQ molecules did not 
      differ between the COPD patients and controls. HLA-DP molecule density seemed to 
      be slightly, but not significantly lower in COPD, whereas surface HLA-DR 
      molecules were significantly reduced (p < 0.005 vs controls). Frequencies of QAP 
      alleles were not different between the COPD patients and controls, but the QBP 
      5.12 allele was significantly more frequent in COPD than in controls (chi 2 = 
      10.83, p = 0.0182, RR 5.5). In conclusion, individuals with exacerbated chronic 
      obstructive pulmonary disease have reduced surface DR molecule expression and an 
      increased frequency of the QBP 5.12 allele. The possible relationship between 
      these two features and the possible role of cytokines in reducing human leukocyte 
      antigen-DR expression in exacerbated chronic obstructive pulmonary disease is 
      explored.
FAU - Recalde, H
AU  - Recalde H
AD  - Centro Trasfusionale, Ospedale Fornaroli, Magenta, Italy.
FAU - Cuccia, M
AU  - Cuccia M
FAU - Oggionni, T
AU  - Oggionni T
FAU - Dondi, E
AU  - Dondi E
FAU - Martinetti, M
AU  - Martinetti M
FAU - Luisetti, M
AU  - Luisetti M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Monaldi Arch Chest Dis
JT  - Monaldi archives for chest disease = Archivio Monaldi per le malattie del torace
JID - 9307314
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Histocompatibility Antigens Class I/analysis
MH  - Histocompatibility Antigens Class II/*analysis/genetics
MH  - Humans
MH  - Lung Diseases, Obstructive/*immunology
MH  - Lymphocytes/*immunology
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic
EDAT- 2000/03/31 09:00
MHDA- 2000/04/15 09:00
CRDT- 2000/03/31 09:00
PHST- 2000/03/31 09:00 [pubmed]
PHST- 2000/04/15 09:00 [medline]
PHST- 2000/03/31 09:00 [entrez]
PST - ppublish
SO  - Monaldi Arch Chest Dis. 1999 Oct;54(5):384-9.