PMID- 10741805
OWN - NLM
STAT- MEDLINE
DCOM- 20000707
LR  - 20171206
IS  - 0391-3988 (Print)
IS  - 0391-3988 (Linking)
VI  - 23
IP  - 2
DP  - 2000 Feb
TI  - Comparative analysis of metabolism of medium- and plasma perfused primary pig 
      hepatocytes cultured around a 3-D membrane network.
PG  - 104-10
AB  - Culture media are frequently used in the evaluation of metabolical functions of 
      hepatocytes in hybrid liver support systems (hLSS). However, media compositions 
      differ substantially from those of plasma. Therefore, our study was designed to 
      investigate whether current in vitro studies with medium are suitable to assess 
      the metabolical competence of hLSS-cultures during clinical application as well 
      as to explore whether the cell nutrition with medium provides a suitable modus 
      operandi for stand by cultivation. Paired bioreactor cultures were perfused with 
      either Williams' Medium E (MPB) or human plasma (PPB). About 6x108 primary pig 
      hepatocytes (>97% viability) were cultured in three laboratory scale bioreactors 
      designed according to Gerlach's bioreactor-concept. Different perfusion protocols 
      were initiated after a standardised period allowing for cell attachment and 
      reorganisation in aggregates. Whereas patterns of enzyme release were similar in 
      both protocols the metabolical behaviour was different between MPB (anabolic 
      state) and PPB (catabolic state). Furthermore, compared to MPB the 
      lidocaine-MEGX-tests for PPB demonstrated lower MEGX-concentrations and a 
      different reaction pattern. We conclude that the nutrition of hepatocytes with 
      medium during the stand by period itself might influence the cell function and 
      subsequently the efficacy of the hLSS-treatment during clinical application.
FAU - Unger, J K
AU  - Unger JK
AD  - Department of Anaesthesiology, Rheinisch-Westfalische Technische Hochschule, 
      Aachen, Germany.
FAU - Catapano, G
AU  - Catapano G
FAU - Horn, N A
AU  - Horn NA
FAU - Schroers, A
AU  - Schroers A
FAU - Gerlach, J C
AU  - Gerlach JC
FAU - Rossaint, R
AU  - Rossaint R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Artif Organs
JT  - The International journal of artificial organs
JID - 7802649
RN  - 0 (Culture Media)
SB  - IM
MH  - Animals
MH  - *Artificial Organs
MH  - Capillary Permeability/physiology
MH  - Cell Aggregation/physiology
MH  - Cells, Cultured
MH  - *Culture Media
MH  - Liver/cytology/enzymology/*metabolism
MH  - Male
MH  - Perfusion/instrumentation
MH  - Swine
EDAT- 2000/03/31 09:00
MHDA- 2000/07/15 11:00
CRDT- 2000/03/31 09:00
PHST- 2000/03/31 09:00 [pubmed]
PHST- 2000/07/15 11:00 [medline]
PHST- 2000/03/31 09:00 [entrez]
PST - ppublish
SO  - Int J Artif Organs. 2000 Feb;23(2):104-10.