PMID- 10744627
OWN - NLM
STAT- MEDLINE
DCOM- 20000501
LR  - 20190516
IS  - 0892-6638 (Print)
IS  - 0892-6638 (Linking)
VI  - 14
IP  - 5
DP  - 2000 Apr
TI  - Occurrence of D-aspartic acid and N-methyl-D-aspartic acid in rat neuroendocrine 
      tissues and their role in the modulation of luteinizing hormone and growth 
      hormone release.
PG  - 699-714
AB  - Using two specific and sensitive fluorometric/HPLC methods and a GC-MS method, 
      alone and in combination with D-aspartate oxidase, we have demonstrated for the 
      first time that N-methyl-D-aspartate (NMDA), in addition to D-aspartate (D-Asp), 
      is endogenously present as a natural molecule in rat nervous system and endocrine 
      glands. Both of these amino acids are mostly concentrated at nmol/g levels in the 
      adenohypophysis, hypothalamus, brain, and testis. The adenohypophysis maximally 
      showed the ability to accumulate D-Asp when the latter is exogenously 
      administered. In vivo experiments, consisting of the i.p. injection of D-Asp, 
      showed that D-Asp induced both growth hormone and luteinizing hormone (LH) 
      release. However, in vitro experiments showed that D-Asp was able to induce LH 
      release from adenohypophysis only when this gland was co-incubated with the 
      hypothalamus. This is because D-Asp also induces the release of GnRH from the 
      hypothalamus, which in turn is directly responsible for the D-Asp-induced LH 
      secretion from the pituitary gland. Compared to D-Asp, NMDA elicits its hormone 
      release action at concentrations approximately 100-fold lower than D-Asp. D-AP5, 
      a specific NMDA receptor antagonist, inhibited D-Asp and NMDA hormonal activity, 
      demonstrating that these actions are mediated by NMDA receptors. NMDA is 
      biosynthesized from D-Asp by an S-adenosylmethionine-dependent enzyme, which we 
      tentatively denominated as NMDA synthase.
FAU - D'Aniello, A
AU  - D'Aniello A
AD  - Department of Biochemistry and Molecular Biology and Neurobiology, Zoological 
      Station 'A. Dohrn', 80121, Napoli, Italy. daniello@alpha.snz.it
FAU - Di Fiore, M M
AU  - Di Fiore MM
FAU - Fisher, G H
AU  - Fisher GH
FAU - Milone, A
AU  - Milone A
FAU - Seleni, A
AU  - Seleni A
FAU - D'Aniello, S
AU  - D'Aniello S
FAU - Perna, A F
AU  - Perna AF
FAU - Ingrosso, D
AU  - Ingrosso D
LA  - eng
GR  - GM TW00033/GM/NIGMS NIH HHS/United States
GR  - SO6GM45455/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9002-72-6 (Growth Hormone)
SB  - IM
MH  - Animals
MH  - Aspartic Acid/*metabolism/pharmacology
MH  - Circadian Rhythm
MH  - Gonadotropin-Releasing Hormone/metabolism
MH  - Growth Hormone/*metabolism
MH  - Hypothalamus/drug effects/physiology
MH  - In Vitro Techniques
MH  - Luteinizing Hormone/*metabolism
MH  - Male
MH  - N-Methylaspartate/*metabolism/pharmacology
MH  - Neurosecretory Systems/drug effects/*physiology
MH  - Pituitary Gland, Anterior/drug effects/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Distribution
EDAT- 2000/04/01 09:00
MHDA- 2000/05/08 09:00
CRDT- 2000/04/01 09:00
PHST- 2000/04/01 09:00 [pubmed]
PHST- 2000/05/08 09:00 [medline]
PHST- 2000/04/01 09:00 [entrez]
AID - 10.1096/fasebj.14.5.699 [doi]
PST - ppublish
SO  - FASEB J. 2000 Apr;14(5):699-714. doi: 10.1096/fasebj.14.5.699.