PMID- 10747014
OWN - NLM
STAT- MEDLINE
DCOM- 20000515
LR  - 20191210
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 19
IP  - 7
DP  - 2000 Apr 3
TI  - gC1q-R/p32, a C1q-binding protein, is a receptor for the InlB invasion protein of 
      Listeria monocytogenes.
PG  - 1458-66
AB  - InlB is a Listeria monocytogenes protein that promotes entry of the bacterium 
      into mammalian cells by stimulating tyrosine phosphorylation of the adaptor 
      proteins Gab1, Cbl and Shc, and activation of phosphatidyl- inositol (PI) 
      3-kinase. Using affinity chromatography and enzyme-linked immunosorbent assay, we 
      demonstrate a direct interaction between InlB and the mammalian protein gC1q-R, 
      the receptor of the globular part of the complement component C1q. Soluble C1q or 
      anti-gC1q-R antibodies impair InlB-mediated entry. Transient transfection of 
      GPC16 cells, which are non-permissive to InlB-mediated entry, with a 
      plasmid-expressing human gC1q-R promotes entry of InlB-coated beads. Furthermore, 
      several experiments indicate that membrane recruitment and activation of PI 
      3-kinase involve an InlB-gC1q-R interaction and that gC1q-R associates with Gab1 
      upon stimulation of Vero cells with InlB. Thus, gC1q-R constitutes a cellular 
      receptor involved in InlB-mediated activation of PI 3-kinase and tyrosine 
      phosphorylation of the adaptor protein Gab1. After E-cadherin, the receptor for 
      internalin, gC1q-R is the second identified mammalian receptor promoting entry of 
      L. monocytogenes into mammalian cells.
FAU - Braun, L
AU  - Braun L
AD  - Unite des Interactions Bacteries-Cellules, Institut Pasteur, 28 rue du Docteur 
      Roux, 75724 Paris cedex 15, France.
FAU - Ghebrehiwet, B
AU  - Ghebrehiwet B
FAU - Cossart, P
AU  - Cossart P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antibodies)
RN  - 0 (Bacterial Proteins)
RN  - 0 (C1QBP protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (GAB1 protein, human)
RN  - 0 (Hyaluronan Receptors)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, Complement)
RN  - 0 (complement 1q receptor)
RN  - 0 (inlB protein, Listeria monocytogenes)
RN  - 42HK56048U (Tyrosine)
RN  - 80295-33-6 (Complement C1q)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Bacterial Proteins/genetics/*metabolism
MH  - Base Sequence
MH  - Binding, Competitive
MH  - Carrier Proteins
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Complement C1q/*metabolism
MH  - DNA Primers/genetics
MH  - HeLa Cells
MH  - Humans
MH  - *Hyaluronan Receptors
MH  - Listeria monocytogenes/genetics/*metabolism/pathogenicity
MH  - *Membrane Glycoproteins
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mitochondrial Proteins
MH  - Molecular Sequence Data
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation
MH  - Receptors, Complement/antagonists & inhibitors/genetics/*metabolism
MH  - Transfection
MH  - Tyrosine/metabolism
MH  - Vero Cells
PMC - PMC310215
EDAT- 2000/04/04 09:00
MHDA- 2000/05/20 09:00
PMCR- 2001/04/03
CRDT- 2000/04/04 09:00
PHST- 2000/04/04 09:00 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 2000/04/04 09:00 [entrez]
PHST- 2001/04/03 00:00 [pmc-release]
AID - 191458 [pii]
AID - 10.1093/emboj/19.7.1458 [doi]
PST - ppublish
SO  - EMBO J. 2000 Apr 3;19(7):1458-66. doi: 10.1093/emboj/19.7.1458.