PMID- 10747192
OWN - NLM
STAT- MEDLINE
DCOM- 20000602
LR  - 20240322
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 524 Pt 1
IP  - Pt 1
DP  - 2000 Apr 1
TI  - Brain-derived neurotrophic factor blocks long-term depression in solitary 
      neurones cultured from rat visual cortex.
PG  - 195-204
AB  - 1. To address questions of whether long-term depression (LTD) in the visual 
      cortex is expressed in pre- or postsynaptic sites, whether brain-derived 
      neurotrophic factor (BDNF) exerts its LTD-blocking action without involvement of 
      GABAergic inhibition, and whether the action of BDNF is pre- or postsynaptic, we 
      observed excitatory postsynaptic currents (EPSCs) from solitary neurones cultured 
      on glial microislands. In this preparation GABAergic inhibition is not involved 
      and a group of synapses (autapses) which generate evoked EPSCs is thought to be 
      the same as those generating spontaneous EPSCs. 2. A short depolarising voltage 
      step to the soma generated Na+ spikes which were followed by autaptic EPSCs. When 
      this somatic activation was paired with prolonged depolarisation for 100 ms to 
      -30 mV and repeated at 1 Hz for 5 min, LTD was induced in all of the nine cells 
      tested. Then, the frequency of spontaneous EPSCs decreased, but the amplitude did 
      not change, suggesting that the site of LTD expression is presynaptic. 3. 
      Application of BDNF at 50 ng ml-1 blocked the depression of evoked EPSCs and the 
      decrease in the frequency of spontaneous EPSCs. An inhibitor for receptor 
      tyrosine kinases, K252a, antagonised the action of BDNF, suggesting an 
      involvement of BDNF receptors, TrkB. 4. These results suggest that BDNF prevents 
      low-frequency inputs from inducing LTD of excitatory synaptic transmission 
      through presynaptic mechanisms in the developing visual cortex.
FAU - Kumura, E
AU  - Kumura E
AD  - CREST, Japan Science and Technology Corporation and Division of Neurophysiology, 
      Biomedical Research Center, Osaka University Graduate School of Medicine, 2-2 
      Yamadaoka, Suita, 565-0871 Japan.
FAU - Kimura, F
AU  - Kimura F
FAU - Taniguchi, N
AU  - Taniguchi N
FAU - Tsumoto, T
AU  - Tsumoto T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carbazoles)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indole Alkaloids)
RN  - 6OTE87SCCW (6-Cyano-7-nitroquinoxaline-2,3-dione)
RN  - 97161-97-2 (staurosporine aglycone)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - 6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Carbazoles/pharmacology
MH  - Cells, Cultured
MH  - Electric Stimulation
MH  - Enzyme Inhibitors/pharmacology
MH  - Indole Alkaloids
MH  - Neuronal Plasticity/drug effects/*physiology
MH  - Neurons/drug effects/*physiology
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reaction Time
MH  - Receptor Protein-Tyrosine Kinases/antagonists & inhibitors
MH  - Visual Cortex/cytology/*physiology
PMC - PMC2269848
EDAT- 2000/04/04 09:00
MHDA- 2000/06/10 09:00
PMCR- 2001/04/01
CRDT- 2000/04/04 09:00
PHST- 2000/04/04 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/04/04 09:00 [entrez]
PHST- 2001/04/01 00:00 [pmc-release]
AID - PHY_9992 [pii]
AID - 10.1111/j.1469-7793.2000.t01-2-00195.x [doi]
PST - ppublish
SO  - J Physiol. 2000 Apr 1;524 Pt 1(Pt 1):195-204. doi: 
      10.1111/j.1469-7793.2000.t01-2-00195.x.