PMID- 10747885
OWN - NLM
STAT- MEDLINE
DCOM- 20000711
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 22
DP  - 2000 Jun 2
TI  - Stimulation of DNA synthesis and cell proliferation of human mammary 
      myoepithelial-like cells by hepatocyte growth factor/scatter factor depends on 
      heparan sulfate proteoglycans and sustained phosphorylation of mitogen-activated 
      protein kinases p42/44.
PG  - 17094-9
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a heparan/dermatan 
      sulfate-binding growth factor produced by stromal cells that acts as a paracrine 
      effector on neighboring epithelia. HGF/SF stimulated DNA synthesis in human 
      mammary (Huma) 109 myoepithelial-like cells grown on collagen I and fibronectin 
      substrata but not when grown on plastic. Dual phosphorylation of 
      mitogen-activated protein kinases (p42/44(MAPK)) was required for this 
      stimulation of DNA synthesis. In Huma 109 cells cultured on plastic, HGF/SF 
      stimulated a transient phosphorylation of p42/44(MAPK), which reached a maximum 
      at 10 min after addition of the growth factor and returned to near basal levels 
      after 20 min. In contrast, the phosphorylation of p42/44(MAPK) stimulated by 
      HGF/SF in cells cultured on collagen I or fibronectin was sustained over 45 min. 
      In Huma 109 cells deficient in sulfated glycosaminoglycans, HGF/SF failed to 
      stimulate p42/44(MAPK) phosphorylation or DNA synthesis on any substratum, even 
      when soluble heparan sulfate proteoglycans purified from the cells or from the 
      culture medium were added. However, HGF/SF stimulated DNA synthesis and a 
      sustained phosphorylation of p42/44(MAPK) in sulfated glycosaminoglycan-deficient 
      Huma 109 cells plated on a substratum of medium HSPGs but not cell HSPGs. The 
      HGF/SF-induced proliferation is thus highly dependent on heparan sulfate 
      proteoglycans in myoepithelial-like cells.
FAU - Sergeant, N
AU  - Sergeant N
AD  - School of Biological Sciences, University of Liverpool, Liverpool L69 7ZB, 
      University of Manchester, Christie Hospital NHS Trust, Wilmslow Road, Manchester 
      M20 4BX, United Kingdom.
FAU - Lyon, M
AU  - Lyon M
FAU - Rudland, P S
AU  - Rudland PS
FAU - Fernig, D G
AU  - Fernig DG
FAU - Delehedde, M
AU  - Delehedde M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Fibronectins)
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Breast/*cytology
MH  - Cell Division/*physiology
MH  - Cells, Cultured
MH  - Collagen/metabolism
MH  - DNA Replication/*physiology
MH  - Fibronectins/metabolism
MH  - Heparan Sulfate Proteoglycans/*physiology
MH  - Hepatocyte Growth Factor/*physiology
MH  - Humans
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Phosphorylation
EDAT- 2000/04/05 09:00
MHDA- 2000/07/15 11:00
CRDT- 2000/04/05 09:00
PHST- 2000/04/05 09:00 [pubmed]
PHST- 2000/07/15 11:00 [medline]
PHST- 2000/04/05 09:00 [entrez]
AID - S0021-9258(19)80316-2 [pii]
AID - 10.1074/jbc.M000237200 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jun 2;275(22):17094-9. doi: 10.1074/jbc.M000237200.