PMID- 10748183
OWN - NLM
STAT- MEDLINE
DCOM- 20000711
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 22
DP  - 2000 Jun 2
TI  - Dependence of elevated human leukocyte antigen class I molecule expression on 
      increased heavy chain, light chain (beta 2-microglobulin), transporter associated 
      with antigen processing, tapasin, and peptide.
PG  - 16643-9
AB  - Human leukocyte antigen (HLA) class I molecule expression was investigated by 
      DNA-mediated gene transfer. Cell surface expression was increased up to 75% by 
      transfection of HLA-A2 or HLA-B8 heavy chain genes but not genes encoding light 
      chains (beta(2)-microglobulin (beta(2)m)), transporter associated with antigen 
      processing (TAP), or tapasin. Interferon (IFN) treatment further increased 
      expression of transfected heavy chains, suggesting that IFN inducible molecules 
      support heavy chain expression. IFN induces beta(2)m, TAP, and tapasin mRNAs. 
      Transfected heavy chain expression increased upon cotransfection with genes 
      encoding TAP1 and TAP2 but not individual TAP subunits, beta(2)m, or tapasin. 
      Tetracycline inducible heavy chain gene expression was also increased by IFN 
      treatment or TAP cotransfection, suggesting that IFN-induced TAP supports heavy 
      chain maturation. Expression of a mutant that does not interact strongly with 
      TAP, HLA-A2-T134K, was also increased by IFN. Inhibition of TAP-dependent peptide 
      transport by ICP47 reduced heavy chain expression. Expression of HLA-A2, but not 
      HLA-B8, was restored in ICP47 cells by HLA-A2-binding (IP-30) signal peptides. 
      However, these peptides did not further increase transfected HLA-A2 expression, 
      suggesting that peptide availability does not limit heavy chain expression in the 
      absence of ICP47. These results suggest that cytokine-induced TAP supports 
      maturation of HLA class I molecules through combined chaperone and peptide supply 
      functions.
FAU - Johnson, D R
AU  - Johnson DR
AD  - Department of Pathology, Yale University School of Medicine, New Haven, 
      Connecticut 06510, USA. david.johnson@yale.edu
FAU - Mook-Kanamori, B
AU  - Mook-Kanamori B
LA  - eng
GR  - R29-AI35099/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antiporters)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (HLA-B8 Antigen)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (Proteins)
RN  - 0 (beta 2-Microglobulin)
RN  - 0 (tapasin)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.8.- (IFI30 protein, human)
RN  - EC 1.8.- (Oxidoreductases Acting on Sulfur Group Donors)
SB  - IM
MH  - Antiporters/*metabolism
MH  - Base Sequence
MH  - Carrier Proteins/*metabolism
MH  - DNA Primers
MH  - HLA-A2 Antigen/*genetics
MH  - HLA-B8 Antigen/*genetics
MH  - HeLa Cells
MH  - Humans
MH  - Immunoglobulins/*metabolism
MH  - Membrane Transport Proteins
MH  - *Oxidoreductases
MH  - Oxidoreductases Acting on Sulfur Group Donors
MH  - Protein Sorting Signals/*metabolism
MH  - Proteins/metabolism
MH  - Transfection
MH  - beta 2-Microglobulin/*metabolism
EDAT- 2000/04/05 09:00
MHDA- 2000/07/15 11:00
CRDT- 2000/04/05 09:00
PHST- 2000/04/05 09:00 [pubmed]
PHST- 2000/07/15 11:00 [medline]
PHST- 2000/04/05 09:00 [entrez]
AID - S0021-9258(19)80255-7 [pii]
AID - 10.1074/jbc.M910035199 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jun 2;275(22):16643-9. doi: 10.1074/jbc.M910035199.