PMID- 10748289
OWN - NLM
STAT- MEDLINE
DCOM- 20000502
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 118
IP  - 2
DP  - 2000 Apr 15
TI  - Sequential numerical changes of chromosomes 7 and 18 in diffuse-type stomach 
      cancer cell lines: combined comparative genomic hybridization, fluorescence in 
      situ hybridization, and ploidy analyses.
PG  - 99-107
AB  - Sequential changes of chromosomal copy number were analyzed retrospectively in 
      five diffuse-type gastric cancer cell lines by comparative genomic hybridization 
      (CGH), DNA cytometry, and fluorescence in situ hybridization (FISH) with 
      centromeric and painting probes. By CGH, we found loss of 18q21 in all of the 
      cell lines and gains of 7p11-q31, 20q, and 22 in four of the five cell lines. 
      Actual copy numbers of chromosomes 7 and 18 were determined by FISH: disomy 18 
      with (partial) loss of 18q in the two DNA-diploid cell lines (AGS and MKN-45), 
      trisomy 7 in MKN-45, disomy 18 and tetrasomy 7 with one-copy loss of 7p and 
      one-copy gain of 7q tip in DNA-triploid HSC-39/40A, and trisomy 18 and hexasomy 7 
      with one-copy loss of 7q in DNA-tetraploid KATO-III. Because the DNA aneuploidy 
      is thought to result through tetraploidization, and the duplicated chromosomal 
      changes in DNA aneuploid tumors seem to precede tetraploidization, the duplicated 
      gain of chromosome 7 and one-copy loss of 7q in KATO-III were inferred to have 
      occurred before and after tetraploidization, respectively. Similarly, HSC-39/40A 
      were inferred to be preceded by the DNA-diploid stage with disomy 7 and monosomy 
      18. As the loss of 18q21 and the gain of 7p11-q31 were inferred to have occurred 
      already in the DNA diploid stage in at least four and two of the cell lines, 
      respectively, the 18q21 loss may be more important than the 7q gain as an earlier 
      event in the genesis of diffuse-type stomach cancer. The combined CGH, FISH, and 
      ploidy analyses thus give us a clue to extract important earlier events from the 
      chromosomal changes that were screened by CGH alone.
FAU - Okada, K
AU  - Okada K
AD  - First Department of Pathology, Shiga University of Medical Science, Ohtsu, Shiga, 
      Japan.
FAU - Sugihara, H
AU  - Sugihara H
FAU - Bamba, M
AU  - Bamba M
FAU - Bamba, T
AU  - Bamba T
FAU - Hattori, T
AU  - Hattori T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adenocarcinoma/genetics
MH  - Carcinoma, Signet Ring Cell/genetics
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 18
MH  - *Chromosomes, Human, Pair 7
MH  - *Gene Duplication
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Nucleic Acid Hybridization
MH  - Retrospective Studies
MH  - Stomach Neoplasms/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2000/04/05 09:00
MHDA- 2000/05/08 09:00
CRDT- 2000/04/05 09:00
PHST- 2000/04/05 09:00 [pubmed]
PHST- 2000/05/08 09:00 [medline]
PHST- 2000/04/05 09:00 [entrez]
AID - S0165-4608(99)00182-X [pii]
AID - 10.1016/s0165-4608(99)00182-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Apr 15;118(2):99-107. doi: 
      10.1016/s0165-4608(99)00182-x.