PMID- 10748520
OWN - NLM
STAT- MEDLINE
DCOM- 20000508
LR  - 20210105
IS  - 1087-0156 (Print)
IS  - 1087-0156 (Linking)
VI  - 18
IP  - 4
DP  - 2000 Apr
TI  - Induction of human cytotoxic T lymphocytes by artificial antigen-presenting 
      cells.
PG  - 405-9
AB  - The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a 
      promising therapeutic approach for a number of diseases. To overcome the 
      difficulty in generating specific CTLs, we established stable artificial 
      antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any 
      patient of a given human leukocyte antigen (HLA) type. Mouse fibroblasts were 
      retrovirally transduced with a single HLA-peptide complex along with the human 
      accessory molecules B7.1, ICAM-1, and LFA-3. These AAPCs consistently elicit 
      strong stimulation and expansion of HLA-restricted CTLs. Owing to the high 
      efficiency of retrovirus-mediated gene transfer, stable AAPCs can be readily 
      engineered for any HLA molecule and any specific peptide.
FAU - Latouche, J B
AU  - Latouche JB
AD  - Department of Human Genetics Immunology Program, Memorial Sloan-Kettering Cancer 
      Center, New York, NY 10021, USA.
FAU - Sadelain, M
AU  - Sadelain M
LA  - eng
GR  - CA-59350/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Biotechnol
JT  - Nature biotechnology
JID - 9604648
RN  - 0 (CD58 Antigens)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (HLA-B7 Antigen)
RN  - 0 (Recombinant Proteins)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - 3T3 Cells
MH  - Adoptive Transfer
MH  - Animals
MH  - Antigen-Presenting Cells/*immunology
MH  - CD58 Antigens/genetics
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Dendritic Cells/immunology
MH  - Genetic Vectors
MH  - HLA-A2 Antigen/*genetics/immunology
MH  - HLA-B7 Antigen/genetics
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics
MH  - Lymphocyte Activation
MH  - Mice
MH  - Recombinant Proteins/immunology
MH  - Retroviridae
MH  - T-Lymphocytes/*immunology/pathology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Transfection
EDAT- 2000/04/05 09:00
MHDA- 2000/05/16 09:00
CRDT- 2000/04/05 09:00
PHST- 2000/04/05 09:00 [pubmed]
PHST- 2000/05/16 09:00 [medline]
PHST- 2000/04/05 09:00 [entrez]
AID - 10.1038/74455 [doi]
PST - ppublish
SO  - Nat Biotechnol. 2000 Apr;18(4):405-9. doi: 10.1038/74455.