PMID- 10751396
OWN - NLM
STAT- MEDLINE
DCOM- 20000810
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 26
DP  - 2000 Jun 30
TI  - The mouse mammary tumor virus promoter adopts distinct chromatin structures in 
      human breast cancer cells with and without glucocorticoid receptor.
PG  - 20061-8
AB  - Steroid receptors represent a class of transcription regulators that act in part 
      by overcoming the often repressive nature of chromatin to modulate gene activity. 
      The mouse mammary tumor virus (MMTV) promoter is a useful model for studying 
      transcriptional regulation by steroid hormone receptors in the context of 
      chromatin. The chromatin architecture of the promoter prevents the assembly of 
      basal transcription machinery and binding of ubiquitous transcription factors. 
      However, in human breast carcinoma T47D cells lacking the glucocorticoid receptor 
      (GR), but expressing the progesterone receptor (PR), nucleosome B (nuc B) assumes 
      a constitutively hypersensitive chromatin structure. This correlation led us to 
      test the hypothesis that the chromatin structure of nuc B was dependent on GR 
      expression in T47D cells. To examine this possibility, we stably co-transfected 
      the MMTV promoter and the GR into T47D cells that lacked both the GR and the PR. 
      We found that in T47D cells that lack both the GR and the PR or express only the 
      GR, nuc B assumes a constitutively "open" chromatin structure, which allows 
      hormone independent access by restriction endonucleases and transcription 
      factors. These results suggest that in GR(+)/pr(-) T47D cells, the MMTV chromatin 
      structure permits GR transcriptional activation, independent of chromatin 
      remodeling.
FAU - Kinyamu, H K
AU  - Kinyamu HK
AD  - Chromatin and Gene Expression Section, Laboratory of Reproductive and 
      Developmental Toxicology, NIEHS, National Institutes of Health, Research Triangle 
      Park, North Carolina 27709, USA.
FAU - Fryer, C J
AU  - Fryer CJ
FAU - Horwitz, K B
AU  - Horwitz KB
FAU - Archer, T K
AU  - Archer TK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glucocorticoids)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Transcription Factors)
RN  - 0 (Y-Box-Binding Protein 1)
RN  - 0 (YBX1 protein, human)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Blotting, Western
MH  - Breast Neoplasms/*virology
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - Cell Nucleus/metabolism
MH  - Chromatin/chemistry/*metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Dexamethasone/pharmacology
MH  - Glucocorticoids/pharmacology
MH  - Humans
MH  - Hypersensitivity
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - NFI Transcription Factors
MH  - Nuclear Proteins
MH  - Precipitin Tests
MH  - *Promoter Regions, Genetic
MH  - Protein Binding
MH  - Receptors, Glucocorticoid/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Transcription Factors
MH  - Transcription, Genetic
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Y-Box-Binding Protein 1
EDAT- 2000/04/07 09:00
MHDA- 2000/08/12 11:00
CRDT- 2000/04/07 09:00
PHST- 2000/04/07 09:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/04/07 09:00 [entrez]
AID - S0021-9258(19)80087-X [pii]
AID - 10.1074/jbc.M001142200 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jun 30;275(26):20061-8. doi: 10.1074/jbc.M001142200.