PMID- 10751892
OWN - NLM
STAT- MEDLINE
DCOM- 20000502
LR  - 20081121
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 163
IP  - 5
DP  - 2000 May
TI  - Frequent genotype changes at -308, and 488 regions of the tumor necrosis 
      factor-alpha (TNF-alpha) gene in patients with prostate cancer.
PG  - 1584-7
AB  - PURPOSE: Tumor necrosis factor-alpha (TNF-alpha) is involved in oncogenesis of 
      several cancers. The purpose of this study was to investigate whether genotype 
      changes of TNF-alpha promoter regions (-238, -308) and at the 488 region are 
      associated with human prostate cancer. MATERIALS AND METHODS: The DNA from 73 
      cases of human prostate cancer was analyzed by allele-specific polymerase chain 
      reaction to characterize the genotype changes of three regions of the TNF-alpha 
      gene in prostate cancer patients. We also determined the genotype frequency in 
      these patients. The relative risk of variant genotype was calculated by comparing 
      with our previous data from healthy controls. RESULTS: Genetic changes were 
      detected in 15.1% (11/73) of prostate cancer samples at 488 region of TNF-alpha. 
      Seventy-three percent (53/73) of the patients showed genotype GA at -308 region 
      of TNF-alpha. Genotype GA at 488 region in TNF-alpha was observed in 73% (53/73) 
      of the cancer and 71% (52/73) of the normal tissue. The relative risks of 
      incidence for prostate cancer was 14-fold higher in people with genotype GA at 
      -308 region of TNF-alpha. The relative incidence for prostate cancer was a 
      17-fold higher in-patient with genotype GA at 488 region of TNF-alpha. Genotype 
      GA at -308 of TNF-alpha was related to higher clinical tumor stage of prostate 
      cancer than genotype G (p <0.05). CONCLUSIONS: The present study demonstrates, 
      for the first time, that the genotype changes in -308 and 488 regions of 
      TNF-alpha are associated with prostate cancer.
FAU - Oh, B R
AU  - Oh BR
AD  - Department of Urology, Chonnam University Medical School, Kwangju, Korea.
FAU - Sasaki, M
AU  - Sasaki M
FAU - Perinchery, G
AU  - Perinchery G
FAU - Ryu, S B
AU  - Ryu SB
FAU - Park, Y I
AU  - Park YI
FAU - Carroll, P
AU  - Carroll P
FAU - Dahiya, R
AU  - Dahiya R
LA  - eng
GR  - AG16870/AG/NIA NIH HHS/United States
GR  - CA64872/CA/NCI NIH HHS/United States
GR  - DK47517/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Alleles
MH  - *Genotype
MH  - Humans
MH  - Male
MH  - Promoter Regions, Genetic/*genetics
MH  - Prostatic Neoplasms/*genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2001/02/07 11:00
MHDA- 2001/02/07 11:01
CRDT- 2001/02/07 11:00
PHST- 2001/02/07 11:00 [pubmed]
PHST- 2001/02/07 11:01 [medline]
PHST- 2001/02/07 11:00 [entrez]
AID - S0022-5347(05)67683-5 [pii]
PST - ppublish
SO  - J Urol. 2000 May;163(5):1584-7.