PMID- 10752688
OWN - NLM
STAT- MEDLINE
DCOM- 20000428
LR  - 20190915
IS  - 0903-4641 (Print)
IS  - 0903-4641 (Linking)
VI  - 108
IP  - 3
DP  - 2000 Mar
TI  - Absence of mutations in the kinase domain of the Met gene and frequent expression 
      of Met and HGF/SF protein in primary gastric carcinomas.
PG  - 195-200
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) and its receptor, Met, are known 
      to play important roles in tumor cell migration, invasion, and metastasis. We 
      analyzed the expression of these genes and the mutations in the kinase domain of 
      the Met gene in 43 gastric carcinomas. Of the 43 cases, the Met and HGF/SF 
      protein were expressed in 29 (67%) and 22 (51%), respectively. All of the cases 
      with HGF/SF immunopositivity also expressed Met. Of 22 cases with HGF/SF 
      immunopositivity, 13 (59%) expressed HGF/SF in tumor cells as well as 
      fibroblasts. We detected no aberrant single-strand conformational polymorphism 
      patterns, suggesting that there are no genetic alterations in the kinase domain 
      of the Met gene. These results indicate that HGF/SF-mediated autocrine and/or 
      paracrine stimulation and overexpression rather than structural alteration of its 
      receptor may contribute to the development and progression of gastric carcinoma, 
      and that expression of Met and HGF/SF may confer a growth advantage to neoplastic 
      cells.
FAU - Park, W S
AU  - Park WS
AD  - Department of Pathology, College of Medicine, The Catholic University of Korea, 
      Seoul.
FAU - Oh, R R
AU  - Oh RR
FAU - Kim, Y S
AU  - Kim YS
FAU - Park, J Y
AU  - Park JY
FAU - Shin, M S
AU  - Shin MS
FAU - Lee, H K
AU  - Lee HK
FAU - Lee, S H
AU  - Lee SH
FAU - Yoo, N J
AU  - Yoo NJ
FAU - Lee, J Y
AU  - Lee JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Adenocarcinoma/classification/*enzymology/pathology/physiopathology
MH  - Gene Expression
MH  - Hepatocyte Growth Factor/biosynthesis/genetics
MH  - Humans
MH  - *Mutation
MH  - Neoplasm Invasiveness
MH  - Polymorphism, Single-Stranded Conformational
MH  - Proto-Oncogene Proteins c-met/biosynthesis/*genetics
MH  - Stomach Neoplasms/classification/*enzymology/pathology/physiopathology
EDAT- 2001/02/07 11:00
MHDA- 2001/02/07 11:01
CRDT- 2001/02/07 11:00
PHST- 2001/02/07 11:00 [pubmed]
PHST- 2001/02/07 11:01 [medline]
PHST- 2001/02/07 11:00 [entrez]
AID - 10.1034/j.1600-0463.2000.d01-44.x [doi]
PST - ppublish
SO  - APMIS. 2000 Mar;108(3):195-200. doi: 10.1034/j.1600-0463.2000.d01-44.x.