PMID- 10753679
OWN - NLM
STAT- MEDLINE
DCOM- 20000508
LR  - 20041117
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 270
IP  - 2
DP  - 2000 Apr 13
TI  - Replicative forms of TT virus DNA in bone marrow cells.
PG  - 657-62
AB  - TT virus (TTV) is a human virus consisting of a single-stranded, circular DNA 
      genome of 3.8 kilobases (kb). To examine whether TTV replicates in peripheral 
      blood mononuclear cells (PBMCs) and bone marrow cells (BMCs), DNA was extracted 
      from the PBMCs and/or BMCs of six TTV-infected individuals and separated by 
      agarose gel electrophoresis. The TTV DNAs from the PBMCs migrated to the 2.0- to 
      2.5-kb region. The TTV DNAs from the BMCs migrated to the 2.0- to 2. 5-kb and 
      3.3- to 6.1-kb regions. The faster-migrating TTV DNAs were sensitive to S1 
      nuclease, while the slower-migrating TTV DNAs were resistant and their position 
      on the agarose gel shifted to the position of the full genomic size upon 
      digestion with restriction enzyme PstI. Full-length inverted polymerase chain 
      reaction on the slower-migrating, double-stranded TTV DNAs from the BMCs 
      amplified a 3.8-kb product. Replicative intermediate forms of TTV DNA are present 
      in BMCs but not in PBMCs.
CI  - Copyright 2000 Academic Press.
FAU - Okamoto, H
AU  - Okamoto H
AD  - Immunology Division and Division of Molecular Virology, Tochigi-Ken, 329-0498, 
      Japan. immunodiv@jichi.ac.jp
FAU - Takahashi, M
AU  - Takahashi M
FAU - Nishizawa, T
AU  - Nishizawa T
FAU - Tawara, A
AU  - Tawara A
FAU - Sugai, Y
AU  - Sugai Y
FAU - Sai, T
AU  - Sai T
FAU - Tanaka, T
AU  - Tanaka T
FAU - Tsuda, F
AU  - Tsuda F
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - Bone Marrow Cells/*virology
MH  - DNA Primers
MH  - *DNA Replication
MH  - DNA Viruses/*genetics
MH  - DNA, Viral/analysis/*biosynthesis
MH  - Humans
MH  - Polymerase Chain Reaction
EDAT- 2001/02/07 11:00
MHDA- 2001/02/07 11:01
CRDT- 2001/02/07 11:00
PHST- 2001/02/07 11:00 [pubmed]
PHST- 2001/02/07 11:01 [medline]
PHST- 2001/02/07 11:00 [entrez]
AID - S0006-291X(00)92481-1 [pii]
AID - 10.1006/bbrc.2000.2481 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Apr 13;270(2):657-62. doi: 
      10.1006/bbrc.2000.2481.