PMID- 10753727 OWN - NLM STAT- MEDLINE DCOM- 20000601 LR - 20240109 IS - 0022-1899 (Print) IS - 0022-1899 (Linking) VI - 181 IP - 4 DP - 2000 Apr TI - A foodborne outbreak of gastroenteritis associated with Norwalk-like viruses: first molecular traceback to deli sandwiches contaminated during preparation. PG - 1467-70 AB - In March 1998, an outbreak of acute gastroenteritis occurred among students at a Texas university. Overall, 125 ill students sought medical care. Case-control studies revealed that illness was significantly associated with eating foods from the university's main cafeteria deli bar on 9 and 10 March. Stool specimens from 9 (50%) of 18 ill students and samples of deli ham showed evidence of Norwalk-like viruses (NLVs) by reverse-transcriptase (RT) polymerase chain reaction (PCR) assay. A food handler who prepared sandwiches for lunch on 9 March reported that her infant had been sick with watery diarrhea since just before the outbreak. A stool sample from the infant was positive for NLV by RT-PCR, and the sequence of the amplified product was identical to that of amplified product from deli ham and students' stool specimens. This is the first time RT-PCR and sequence analysis have successfully confirmed viral contamination of a food item likely to have been contaminated by a food handler. FAU - Daniels, N A AU - Daniels NA AD - Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, Atlanta, GA 30333, USA. FAU - Bergmire-Sweat, D A AU - Bergmire-Sweat DA FAU - Schwab, K J AU - Schwab KJ FAU - Hendricks, K A AU - Hendricks KA FAU - Reddy, S AU - Reddy S FAU - Rowe, S M AU - Rowe SM FAU - Fankhauser, R L AU - Fankhauser RL FAU - Monroe, S S AU - Monroe SS FAU - Atmar, R L AU - Atmar RL FAU - Glass, R I AU - Glass RI FAU - Mead, P AU - Mead P LA - eng GR - AI-07471/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. DEP - 20000407 PL - United States TA - J Infect Dis JT - The Journal of infectious diseases JID - 0413675 SB - IM MH - Adolescent MH - Adult MH - Caliciviridae Infections/*epidemiology/transmission/*virology MH - Case-Control Studies MH - Diarrhea/virology MH - *Disease Outbreaks MH - Feces/microbiology/virology MH - Female MH - Food Handling MH - Foodborne Diseases/*epidemiology/*virology MH - Gastroenteritis/*epidemiology/*virology MH - Humans MH - Infant MH - Male MH - *Norwalk virus MH - Polymerase Chain Reaction MH - Texas MH - Universities EDAT- 2000/04/08 09:00 MHDA- 2000/06/03 09:00 CRDT- 2000/04/08 09:00 PHST- 1999/04/28 00:00 [received] PHST- 1999/09/28 00:00 [revised] PHST- 2000/04/08 09:00 [pubmed] PHST- 2000/06/03 09:00 [medline] PHST- 2000/04/08 09:00 [entrez] AID - JID990520 [pii] AID - 10.1086/315365 [doi] PST - ppublish SO - J Infect Dis. 2000 Apr;181(4):1467-70. doi: 10.1086/315365. Epub 2000 Apr 7.