PMID- 10753852
OWN - NLM
STAT- MEDLINE
DCOM- 20000505
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 95
IP  - 8
DP  - 2000 Apr 15
TI  - Concurrent activation of a novel putative transforming gene, myeov, and cyclin D1 
      in a subset of multiple myeloma cell lines with t(11;14)(q13;q32).
PG  - 2691-8
AB  - Through the application of the NIH/3T3 tumorigenicity assay to DNA from a gastric 
      carcinoma, we have identified a novel transforming gene, designated myeov 
      (myeloma overexpressed gene in a subset of t[11;14]-positive multiple myelomas). 
      Sequence analyses did not reveal any homology with sequences present in the 
      GenBank, except the deduced protein structure predicts a transmembrane 
      localization. Myeov was mapped to chromosome 11q13 and localized by DNA fiber 
      fluorescence in situ hybridization (FISH) 360-kilobase (kb) centromeric of cyclin 
      D1. In 3 of 7 multiple myeloma (MM) cell lines with a t(11;14)(q13;q32) and 
      cyclin-D1 overexpression, Northern blot analysis revealed overexpression of myeov 
      as well. In all 7 cell lines, the translocation breakpoint was mapped within the 
      360-kb region between myeov and cyclin D1. DNA fiber FISH with a contig of probes 
      covering the constant region of the immunoglobulin heavy chain (IgH) revealed 
      that exclusively in the 3 myeov-overexpressing cell lines (KMS-12, KMS-21, and 
      XG-5), either the 5' E(mu) enhancer or the most telomeric 3' Ealpha enhancer was 
      juxtaposed to myeov. Although cyclin D1 overexpression represents a 
      characteristic feature of all MM cell lines with t(11;14), our results 
      demonstrate aberrant expression of a second putative oncogene in a subset of 
      these cases, due to juxtaposition to IgH enhancers. The clinical relevance of 
      this dual activation remains to be elucidated. (Blood. 2000;95:2691-2698)
FAU - Janssen, J W
AU  - Janssen JW
AD  - Institute of Human Genetics, University of Heidelberg, Heidelberg, Germany. 
      hans_janssen@med.uni-heidelberg.de
FAU - Vaandrager, J W
AU  - Vaandrager JW
FAU - Heuser, T
AU  - Heuser T
FAU - Jauch, A
AU  - Jauch A
FAU - Kluin, P M
AU  - Kluin PM
FAU - Geelen, E
AU  - Geelen E
FAU - Bergsagel, P L
AU  - Bergsagel PL
FAU - Kuehl, W M
AU  - Kuehl WM
FAU - Drexler, H G
AU  - Drexler HG
FAU - Otsuki, T
AU  - Otsuki T
FAU - Bartram, C R
AU  - Bartram CR
FAU - Schuuring, E
AU  - Schuuring E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Neoplasm Proteins)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 14
MH  - Cyclin D1/*genetics
MH  - DNA, Neoplasm/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Multiple Myeloma/*genetics
MH  - Neoplasm Proteins/genetics
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 2001/02/07 11:00
MHDA- 2001/02/07 11:01
CRDT- 2001/02/07 11:00
PHST- 2001/02/07 11:00 [pubmed]
PHST- 2001/02/07 11:01 [medline]
PHST- 2001/02/07 11:00 [entrez]
AID - S0006-4971(20)64162-3 [pii]
PST - ppublish
SO  - Blood. 2000 Apr 15;95(8):2691-8.