PMID- 10753854
OWN - NLM
STAT- MEDLINE
DCOM- 20000505
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 95
IP  - 8
DP  - 2000 Apr 15
TI  - Survival of donor cells 25 years after intrauterine transfusion.
PG  - 2709-14
AB  - Persistence of donor leukocytes in the circulation of recipients of intrauterine 
      transfusion (IUT) has been observed up to 5 years after birth. The aim of this 
      study was to determine whether transfusions with nonirradiated, 
      nonleukocyte-depleted donor blood during the fetal period resulted in long-term 
      immunomodulation of the recipient. Twenty-four surviving IUT recipients between 
      1966 and 1976 were tested for autoimmune disease and autoantibodies at follow-up. 
      Ten had sex-mismatched donors and were therefore informative for chimerism 
      studies using fluorescence in situ hybridization (FISH). Seven female recipients 
      could be tested for chimerism using a Y- chromosome-specific polymerase chain 
      reaction (PCR) because they received at least 1 IUT from a male donor. Nine 
      recipients could be studied for cytotoxic T-lymphocyte precursor (CTLp) and 
      helper T-lymphocyte precursor (HTLp) frequencies because the original donors were 
      available for testing. All surviving IUT recipients were in good health at the 
      time of the examination, and routine laboratory testing revealed no 
      abnormalities. None of the IUT recipients were chimeric as determined by FISH 
      analysis, but Y-chromosome-specific sequences were detected by PCR in 6 of the 7 
      women. However, the CTLp and HTLp frequencies of the IUT recipients against the 
      donors were comparable to those of the controls. The current study provides 
      evidence that IUT can result in the persistence of donor cells in the recipient 
      for a period longer than 20 years but that it is not associated with 
      immunotolerance or with signs of chronic antigenic stimulation. (Blood. 
      2000;95:2709-2714)
FAU - Vietor, H E
AU  - Vietor HE
AD  - Department of Obstetrics, Leiden University Medical Center, Leiden, The 
      Netherlands. h.vietor@antrg.azn.nl
FAU - Hallensleben, E
AU  - Hallensleben E
FAU - van Bree, S P
AU  - van Bree SP
FAU - van der Meer, E M
AU  - van der Meer EM
FAU - Kaal, S E
AU  - Kaal SE
FAU - Bennebroek-Gravenhorst, J
AU  - Bennebroek-Gravenhorst J
FAU - Kanhai, H H
AU  - Kanhai HH
FAU - Brand, A
AU  - Brand A
FAU - Claas, F H
AU  - Claas FH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Anemia, Hemolytic, Congenital/immunology/*therapy
MH  - Autoimmunity/*immunology
MH  - *Blood Transfusion, Intrauterine
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Infant
MH  - Male
MH  - Pregnancy
MH  - T-Lymphocytes/immunology
MH  - Time Factors
MH  - Transplantation Chimera
EDAT- 2001/02/07 11:00
MHDA- 2001/02/07 11:01
CRDT- 2001/02/07 11:00
PHST- 2001/02/07 11:00 [pubmed]
PHST- 2001/02/07 11:01 [medline]
PHST- 2001/02/07 11:00 [entrez]
AID - S0006-4971(20)64164-7 [pii]
PST - ppublish
SO  - Blood. 2000 Apr 15;95(8):2709-14.