PMID- 10756053
OWN - NLM
STAT- MEDLINE
DCOM- 20000518
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 9
DP  - 2000 May
TI  - Consequences of Fas-mediated human dendritic cell apoptosis induced by measles 
      virus.
PG  - 4387-93
AB  - Mortality from measles virus (MV) infection is caused mostly by secondary 
      infections associated with a pronounced immunosuppression. Dendritic cells (DCs) 
      represent a major target of MV and could be involved in immunosuppression. In 
      this study, human monocyte-derived DCs were used to demonstrate that DC apoptosis 
      in MV-infected DC-T-cell cocultures is Fas mediated, whereas apoptotic T cells 
      could not be rescued by blocking the Fas pathway. Two novel consequences of DC 
      apoptosis after MV infection were demonstrated. (i) Fas-mediated apoptosis of DCs 
      facilitates MV release, while CD40 activation enhances MV replication in DCs. 
      Indeed, detailed studies of infectious MV release and intracellular MV 
      nucleoprotein (NP) showed that inhibition of CD40-CD40L ligand interaction blocks 
      NP synthesis. We conclude that the CD40 ligand expressed by activated T cells 
      first enhances MV replication in DCs, and then Fas ligand produced by activated T 
      cells induces Fas-mediated apoptosis of DCs, thus facilitating MV release. (ii) 
      Not only MV-infected DCs but also bystander uninfected DCs undergo a maturation 
      process confirmed by CD1a, CD40, CD80, CD86, CD83, and major histocompatibility 
      complex type II labeling. The bystander maturation effect results from contact 
      and/or engulfment of MV-induced apoptotic DCs by uninfected DCs. A model is 
      proposed to explain how both a specific immune response and immunosuppression can 
      simultaneously occur after MV infection through Fas-mediated apoptosis and CD40 
      activation of DCs.
FAU - Servet-Delprat, C
AU  - Servet-Delprat C
AD  - Immunobiologie Fondamentale et Clinique, INSERM U503, ENS Lyon, 69 364 Lyon cedex 
      07, France. cservet@ens-lyon.fr
FAU - Vidalain, P O
AU  - Vidalain PO
FAU - Azocar, O
AU  - Azocar O
FAU - Le Deist, F
AU  - Le Deist F
FAU - Fischer, A
AU  - Fischer A
FAU - Rabourdin-Combe, C
AU  - Rabourdin-Combe C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (CD40 Antigens)
RN  - 0 (fas Receptor)
SB  - IM
MH  - *Apoptosis
MH  - CD40 Antigens/immunology
MH  - Coculture Techniques
MH  - Dendritic Cells/cytology/immunology/*virology
MH  - Humans
MH  - Measles virus/immunology/*physiology
MH  - T-Lymphocytes/immunology/virology
MH  - Virus Replication
MH  - fas Receptor/*metabolism
PMC - PMC111955
EDAT- 2001/02/07 11:00
MHDA- 2001/02/07 11:01
PMCR- 2000/05/01
CRDT- 2001/02/07 11:00
PHST- 2001/02/07 11:00 [pubmed]
PHST- 2001/02/07 11:01 [medline]
PHST- 2001/02/07 11:00 [entrez]
PHST- 2000/05/01 00:00 [pmc-release]
AID - 1669 [pii]
AID - 10.1128/jvi.74.9.4387-4393.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 May;74(9):4387-93. doi: 10.1128/jvi.74.9.4387-4393.2000.