PMID- 10756077
OWN - NLM
STAT- MEDLINE
DCOM- 20000609
LR  - 20071114
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 30
IP  - 3
DP  - 2000 May
TI  - Interleukin-1beta-induced expression of monocyte chemotactic protein-1 in the 
      rabbit retina: an in situ and immunohistochemical study.
PG  - 279-89
AB  - In this study, the temporal and spatial expression of the chemotactic factor 
      monocyte chemotactic protein-1 (MCP-1) was examined in the rabbit retina after 
      challenge with the proinflammatory cytokine interleukin-1beta (IL-1). In these 
      tissues, IL-1 induces an acute inflammatory response of the epiretinal vessels 
      that peaks approximately 24 h postintraocular injection (pi) with the cytokine. 
      At 2 h after challenge with IL-1, MCP-1 mRNA was expressed by perivascular 
      microglial cells and astrocytes that form the glial limitans. Protein analysis at 
      3 h pi with IL-1 confirmed these sites of MCP-1 expression. The intensity of the 
      mRNA and protein signals increased at 6 h and at 24 h. At these time points, 
      MCP-1 message and protein also were detected in infiltrating macrophages and, at 
      the latest time point, in endothelial cells as well. These data support the 
      conclusion that IL-1 provides a strong stimulus for the rapid expression of MCP-1 
      mRNA and protein in retinal tissues, and they further support the role of 
      endogenous glial cells as important sources of mediators involved in the 
      regulation of inflammation occurring within the nervous system.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Cuff, C A
AU  - Cuff CA
AD  - Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, 
      USA. ccuff@wista.wistar.upenn.edu
FAU - Berman, J W
AU  - Berman JW
FAU - Brosnan, C F
AU  - Brosnan CF
LA  - eng
GR  - MH52974/MH/NIMH NIH HHS/United States
GR  - NS 07098/NS/NINDS NIH HHS/United States
GR  - NS 11920/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Female
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Interleukin-1/*metabolism
MH  - RNA, Messenger/analysis
MH  - Rabbits
MH  - Retina/*metabolism
MH  - Time Factors
EDAT- 2000/04/08 09:00
MHDA- 2000/06/17 09:00
CRDT- 2000/04/08 09:00
PHST- 2000/04/08 09:00 [pubmed]
PHST- 2000/06/17 09:00 [medline]
PHST- 2000/04/08 09:00 [entrez]
AID - 10.1002/(SICI)1098-1136(200005)30:3<279::AID-GLIA7>3.0.CO;2-6 [pii]
PST - ppublish
SO  - Glia. 2000 May;30(3):279-89.