PMID- 10760046
OWN - NLM
STAT- MEDLINE
DCOM- 20000606
LR  - 20191103
IS  - 1352-0504 (Print)
IS  - 1352-0504 (Linking)
VI  - 7
IP  - 2
DP  - 2000 Mar
TI  - Hepatitis C virus mixed genotype infection in patients on haemodialysis.
PG  - 153-60
AB  - Hepatitis C virus (HCV) has been classified into different genotypes/subtypes 
      with demonstrated clinical implications. Whether there is biological difference 
      between genotypes is unknown. We determined HCV genotype in 120 anti-HCV-positive 
      patients with end-stage renal disease and on haemodialysis, by both serological 
      assay (which showed evidence of previous exposure) and by two molecular assays: 
      restriction fragment length polymorphism (RFLP) and line-probe reverse 
      hybridization (LiPA). In mixing experiments, RFLP and LiPA was able to detect the 
      minor HCV genotypes (if present) in 5-30% and 1-2% of the viral population, 
      respectively. Of the 120 patients studied, genotype-specific antibodies were 
      detected in 50 (42%), and eight patients had reactivities to peptides derived 
      from multiple genotypes (genotypes 1 and 2 and/or 3). Only genotype 1 infection 
      was found by RFLP/LiPA in these eight patients with reactivities to multiple HCV 
      genotypes. One-hundred and five of the 120 (88%) patients were positive for HCV 
      RNA by reverse transcription-polymerase chain reaction (RT-PCR) analysis and 14 
      were found to have mixed genotype infection. Follow-up serum samples (4-21 months 
      later) were available in five patients (genotype 1a with another 
      genotype/subtype). All five patients had a reduced number of HCV genotypes 
      detected during follow-up; four of the five patients still had detectable 
      genotype 1a, and one patient lost genotype 1a and was positive for genotype 2b 
      only. These data showed that HCV mixed infection can be reliably detected by 
      molecular methods and, in patients with end-stage renal disease and mixed 
      genotype infection, there is a trend that during follow-up, HCV genotype 1 may 
      prevail, or 'take over' the genotype 2 and 3 infection.
FAU - Qian, K P
AU  - Qian KP
AD  - Section of Hepatobiliary Diseases, Division of Gastroenterology, Hepatology, and 
      Nutrition, Department of Medicine, University of Florida, Gainesville, FL 32610, 
      USA.
FAU - Natov, S N
AU  - Natov SN
FAU - Pereira, B J
AU  - Pereira BJ
FAU - Lau, J Y
AU  - Lau JY
LA  - eng
GR  - R01 AI41219/AI/NIAID NIH HHS/United States
GR  - R29 DK48876/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Viral Hepat
JT  - Journal of viral hepatitis
JID - 9435672
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Cloning, Molecular
MH  - Cross-Sectional Studies
MH  - DNA, Viral/chemistry
MH  - Genotype
MH  - Hepacivirus/*genetics
MH  - Hepatitis C, Chronic/*virology
MH  - Humans
MH  - Longitudinal Studies
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Renal Dialysis/*adverse effects
EDAT- 2000/04/12 09:00
MHDA- 2000/06/10 09:00
CRDT- 2000/04/12 09:00
PHST- 2000/04/12 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/04/12 09:00 [entrez]
AID - jvh208 [pii]
AID - 10.1046/j.1365-2893.2000.00208.x [doi]
PST - ppublish
SO  - J Viral Hepat. 2000 Mar;7(2):153-60. doi: 10.1046/j.1365-2893.2000.00208.x.