PMID- 10761702
OWN - NLM
STAT- MEDLINE
DCOM- 20000420
LR  - 20190513
IS  - 0910-5050 (Print)
IS  - 1876-4673 (Electronic)
IS  - 0910-5050 (Linking)
VI  - 91
IP  - 2
DP  - 2000 Feb
TI  - Molecular cytogenetic analysis of 17 renal cancer cell lines: increased copy 
      number at 5q31-33 in cell lines from nonpapillary carcinomas.
PG  - 156-63
AB  - Comparative genomic hybridization (CGH) was used to screen for genomic imbalances 
      in cell lines derived from 13 nonpapillary renal-cell carcinomas (RCCs), two 
      papillary RCCs, one renal squamous-cell carcinoma, and one transitional-cell 
      carcinoma of the renal pelvis. Aberrations were found in all 17 lines. The most 
      frequent changes in nonpapillary RCC cell lines were gains of 5q (85%), 7q (69%), 
      8q (69%) and 1q (54%) and losses of 3p (92%), 8p (77%), 4q (62%) and 14q (54%). 
      High-level gains (HLGs) were detected at 4q12, 5p, 5q23-33, 7q22-qter, 8q23-24, 
      10q21-qter, 12p and 12q13-22. By means of fluorescence in situ hybridization 
      (FISH) we narrowed the smallest common region involving 5q gains to the genomic 
      segment between D5S642 and D5S673, and found that the HLG at 4q12 possibly 
      involved amplifications of c-kit and PDGFRA. Two papillary RCC cell lines showed 
      gains of entire chromosomes 7, 12 and 17. The CGH data reported here should help 
      to facilitate the choice of individual renal-tumor cell lines for exploring 
      target genes in regions of interest.
FAU - Yang, Z Q
AU  - Yang ZQ
AD  - Department of Molecular Cytogenetics, Medical Research Institute, Tokyo Medical 
      and Dental University.
FAU - Yoshida, M A
AU  - Yoshida MA
FAU - Fukuda, Y
AU  - Fukuda Y
FAU - Kurihara, N
AU  - Kurihara N
FAU - Nakamura, Y
AU  - Nakamura Y
FAU - Inazawa, J
AU  - Inazawa J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - 0 (DNA, Neoplasm)
SB  - IM
EIN - Jpn J Cancer Res 2000 Jul;91(7):767
MH  - Adult
MH  - Aged
MH  - Carcinoma, Renal Cell/genetics
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - *Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
MH  - Tumor Cells, Cultured
PMC - PMC5926323
EDAT- 2000/04/13 09:00
MHDA- 2000/04/25 09:00
PMCR- 2000/02/01
CRDT- 2000/04/13 09:00
PHST- 2000/04/13 09:00 [pubmed]
PHST- 2000/04/25 09:00 [medline]
PHST- 2000/04/13 09:00 [entrez]
PHST- 2000/02/01 00:00 [pmc-release]
AID - CAE156 [pii]
AID - 10.1111/j.1349-7006.2000.tb00927.x [doi]
PST - ppublish
SO  - Jpn J Cancer Res. 2000 Feb;91(2):156-63. doi: 10.1111/j.1349-7006.2000.tb00927.x.