PMID- 10761710
OWN - NLM
STAT- MEDLINE
DCOM- 20000420
LR  - 20190513
IS  - 0910-5050 (Print)
IS  - 1876-4673 (Electronic)
IS  - 0910-5050 (Linking)
VI  - 91
IP  - 2
DP  - 2000 Feb
TI  - Transforming growth factor-alpha induces the differentiation of sarcomatoid 
      cholangiocarcinoma cells.
PG  - 223-30
AB  - A sarcomatoid cholangiocarcinoma cell line, ETK-1, was established from a 
      patient. Phenotypically, the cells corresponded to immature biliary epithelial 
      cells. Because a small number of ETK-1 cells appeared to differentiate 
      spontaneously along a biliary epithelial lineage in continuous culture, we 
      examined the factors that initiate and/or promote the differentiation of the 
      cells. Transforming growth factor-alpha (TGFalpha) induced significant changes in 
      ETK-1 cells. After stimulation with the factor, ETK-1 cells displayed morphologic 
      transformation at a much higher frequency, with the appearance of many large 
      cells with intracytoplasmic vacuoles, and the production of mucinous substances. 
      These morphologically transformed cells were phenotypically similar to 
      well-differentiated adenocarcinoma cells. The expression pattern of integrins 
      after TGFalpha treatment also supported the maturation of the ETK-1 cells. The 
      antibody against the receptor of TGFalpha inhibited these changes by TGFalpha. 
      Moreover, the proliferation rate of ETK-1 cells was suppressed by TGFalpha. Our 
      data suggest that TGFalpha can act as a differentiation factor along a biliary 
      epithelial lineage.
FAU - Enjoji, M
AU  - Enjoji M
AD  - Department of Medicine and Bioregulatory Science, Graduate School of Medical 
      Sciences, Kyushu University, Fukuoka. enjoji@intmed3.med.kyushu-u.ac.jp
FAU - Nakashima, M
AU  - Nakashima M
FAU - Nakamuta, M
AU  - Nakamuta M
FAU - Nawata, H
AU  - Nawata H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - 0 (Integrins)
RN  - 0 (Receptors, Fibroblast Growth Factor)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 104781-85-3 (Fibroblast Growth Factor 1)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Bile Duct Neoplasms/*pathology
MH  - *Bile Ducts, Intrahepatic
MH  - Cell Differentiation/drug effects
MH  - Cholangiocarcinoma/*pathology
MH  - Fibroblast Growth Factor 1/pharmacology
MH  - Humans
MH  - Integrins/analysis
MH  - Proto-Oncogene Proteins c-kit/analysis
MH  - Receptors, Fibroblast Growth Factor/analysis
MH  - Transforming Growth Factor alpha/*pharmacology
MH  - Tumor Cells, Cultured
PMC - PMC5926319
EDAT- 2000/04/13 09:00
MHDA- 2000/04/25 09:00
PMCR- 2000/02/01
CRDT- 2000/04/13 09:00
PHST- 2000/04/13 09:00 [pubmed]
PHST- 2000/04/25 09:00 [medline]
PHST- 2000/04/13 09:00 [entrez]
PHST- 2000/02/01 00:00 [pmc-release]
AID - CAE223 [pii]
AID - 10.1111/j.1349-7006.2000.tb00935.x [doi]
PST - ppublish
SO  - Jpn J Cancer Res. 2000 Feb;91(2):223-30. doi: 10.1111/j.1349-7006.2000.tb00935.x.