PMID- 10762651
OWN - NLM
STAT- MEDLINE
DCOM- 20000525
LR  - 20190516
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 5
IP  - 5
DP  - 2000 May
TI  - New chromosomal breakpoints in non-Hodgkin's lymphomas revealed by spectral 
      karyotyping and G-banding.
PG  - 485-92
AB  - Chromosomal rearrangements in short term cultures from nine cases of 
      non-Hodgkin's lymphomas (NHL) were characterized by G-banding, spectral 
      karyotyping (SKY), and fluorescence in situ hybridization (FISH). Eight of the 
      nine cases showed complex karyotypes with chromosomal aberrations which, in most 
      cases, could not be fully characterized by traditional G-banding analysis alone. 
      Karyotypic abnormalities of special interest were marker chromosomes and 
      chromosomes with added unidentified chromosomal material, as previously 
      non-identified chromosomal translocations were hidden behind these aberrations. 
      SKY and FISH analysis, as a complement to banding analysis, significantly 
      improved the karyotypes in seven of the nine cases and unveiled 21 previously 
      unidentified rearrangements with novel translocation breakpoints. Traditional 
      G-banding alone revealed seven new rearrangements, which were all confirmed by 
      SKY. None of these new aberrations occurred as single clonal rearrangements but 
      as parts of complex karyotypes. Nevertheless, the chromosomal break-point regions 
      identified should be considered as potential hot spots for genes involved in the 
      tumorigenesis of the malignancy.
FAU - Nordgren, A
AU  - Nordgren A
AD  - Department of Molecular Medicine, CMM L8:02, Karolinska Hospital, S-171 76 
      Stockholm, Sweden.
FAU - Sorensen, A G
AU  - Sorensen AG
FAU - Tinggaard-Pedersen, N
AU  - Tinggaard-Pedersen N
FAU - Blennow, E
AU  - Blennow E
FAU - Larsson, C
AU  - Larsson C
FAU - Lagercrantz, S
AU  - Lagercrantz S
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosome Banding/methods
MH  - Chromosome Breakage/*genetics
MH  - Female
MH  - Humans
MH  - Karyotyping/*methods
MH  - Lymphoma, Non-Hodgkin/*genetics
MH  - Male
MH  - Middle Aged
MH  - Spectrum Analysis/methods
EDAT- 2000/04/14 09:00
MHDA- 2000/06/08 09:00
CRDT- 2000/04/14 09:00
PHST- 2000/04/14 09:00 [pubmed]
PHST- 2000/06/08 09:00 [medline]
PHST- 2000/04/14 09:00 [entrez]
AID - 10.3892/ijmm.5.5.485 [doi]
PST - ppublish
SO  - Int J Mol Med. 2000 May;5(5):485-92. doi: 10.3892/ijmm.5.5.485.