PMID- 10762707
OWN - NLM
STAT- MEDLINE
DCOM- 20000605
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 76
IP  - 2
DP  - 2000 Mar 29
TI  - Depletion of a fatty acid-binding protein impairs neurite outgrowth in PC12 
      cells.
PG  - 315-24
AB  - Epithelial fatty acid-binding protein (E-FABP) is up-regulated in rat dorsal root 
      ganglia after sciatic nerve crush and in differentiating neurons during 
      development. The present study investigates the role of E-FABP during nerve 
      growth factor (NGF)-mediated neurite outgrowth in PC12 cells. Undifferentiated 
      PC12 cells express low levels of E-FABP, while NGF triggers a 6- and 8-fold 
      induction of E-FABP mRNA and protein, respectively. Up-regulation of E-FABP mRNA 
      occurs as early as 24 h after NGF treatment and remains highly expressed over the 
      course of several days, corresponding to NGF-mediated neurite outgrowth. 
      Withdrawal of NGF leads to down-regulation of E-FABP mRNA and retraction of 
      neurites. Immunofluorescence microscopy reveals E-FABP immunoreactivity in the 
      perinuclear cytoplasm, neurites and growth cones of NGF-differentiated cells. To 
      examine the role of E-FABP during neurite outgrowth, PC12 cells were transfected 
      with a constitutive antisense E-FABP vector to create the E-FABP-deficient line 
      PC12-AS. By morphometric analysis, PC12-AS cells treated for 2, 4, and 7 days 
      with NGF exhibited significantly decreased neurite expression relative to control 
      (mock-transfected) cells. Taken together, these data indicate that E-FABP is 
      important in normal NGF-mediated neurite outgrowth in PC12 cells, a finding that 
      is consistent with a potential role in axonal development and regeneration.
FAU - Allen, G W
AU  - Allen GW
AD  - Department of Physiology and Pharmacology and Center for Molecular Biology and 
      Gene Therapy, Loma Linda School of Medicine, Loma Linda University, Mortenson 
      Hall 142 LLU, Loma Linda, CA 92350, USA.
FAU - Liu, J W
AU  - Liu JW
FAU - De Leon, M
AU  - De Leon M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Carrier Proteins)
RN  - 0 (Fabp7 protein, rat)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Fatty Acids)
RN  - 0 (Myelin P2 Protein)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/*genetics/physiology
MH  - Cytoplasm/physiology
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Fatty Acids/metabolism
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Kinetics
MH  - Myelin P2 Protein/*genetics/physiology
MH  - *Neoplasm Proteins
MH  - Nerve Growth Factor/*pharmacology
MH  - *Nerve Tissue Proteins
MH  - Neurites/drug effects/*physiology
MH  - PC12 Cells
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Recombinant Proteins/biosynthesis
MH  - Transfection
EDAT- 2000/04/14 09:00
MHDA- 2000/06/10 09:00
CRDT- 2000/04/14 09:00
PHST- 2000/04/14 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/04/14 09:00 [entrez]
AID - S0169328X00000140 [pii]
AID - 10.1016/s0169-328x(00)00014-0 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2000 Mar 29;76(2):315-24. doi: 
      10.1016/s0169-328x(00)00014-0.